大鼠海马 NMDA 诱发的局域环路动力学的经颅光声成像。
Transcranial photoacoustic imaging of NMDA-evoked focal circuit dynamics in the rat hippocampus.
机构信息
Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University, Baltimore, MD, United States of America. Laboratory of Computational Sensing and Robotics, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, United States of America.
出版信息
J Neural Eng. 2020 Apr 8;17(2):025001. doi: 10.1088/1741-2552/ab78ca.
OBJECTIVE
We report the transcranial functional photoacoustic (fPA) neuroimaging of N-methyl-D-aspartate (NMDA) evoked neural activity in the rat hippocampus. Concurrent quantitative electroencephalography (qEEG) and microdialysis were used to record real-time circuit dynamics and excitatory neurotransmitter concentrations, respectively.
APPROACH
We hypothesized that location-specific fPA voltage-sensitive dye (VSD) contrast would identify neural activity changes in the hippocampus which correlate with NMDA-evoked excitatory neurotransmission.
MAIN RESULTS
Transcranial fPA VSD imaging at the contralateral side of the microdialysis probe provided NMDA-evoked VSD responses with positive correlation to extracellular glutamate concentration changes. qEEG validated a wide range of glutamatergic excitation, which culminated in focal seizure activity after a high NMDA dose. We conclude that transcranial fPA VSD imaging can distinguish focal glutamate loads in the rat hippocampus, based on the VSD redistribution mechanism which is sensitive to the electrophysiologic membrane potential.
SIGNIFICANCE
Our results suggest the future utility of this emerging technology in both laboratory and clinical sciences as an innovative functional neuroimaging modality.
目的
我们报告了大鼠海马区 N-甲基-D-天冬氨酸（NMDA）诱发神经活动的颅外功能光声（fPA）神经影像学。同时使用定量脑电图（qEEG）和微透析分别记录实时电路动力学和兴奋性神经递质浓度。
方法
我们假设位置特异性 fPA 电压敏感染料（VSD）对比将识别与 NMDA 诱发的兴奋性神经传递相关的海马区神经活动变化。
主要结果
在微透析探针的对侧进行颅外 fPA VSD 成像，提供了与细胞外谷氨酸浓度变化呈正相关的 NMDA 诱发 VSD 反应。qEEG 验证了广泛的谷氨酸能兴奋，在高 NMDA 剂量后导致局灶性癫痫发作活动。我们得出结论，基于对电生理膜电位敏感的 VSD 重分布机制，颅外 fPA VSD 成像可以区分大鼠海马区的局灶性谷氨酸负荷。
意义
我们的结果表明，这项新兴技术在实验室和临床科学中作为一种创新的功能神经影像学模式具有未来的应用潜力。
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