Sezgin Erdinc, Carugo Dario, Levental Ilya, Stride Eleanor, Eggeling Christian
MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, UK.
Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, 171 65 Stockholm, Sweden.
Membranes (Basel). 2020 Feb 18;10(2):30. doi: 10.3390/membranes10020030.
Model membrane systems are essential tools for the study of biological processes in a simplified setting to reveal the underlying physicochemical principles. As cell-derived membrane systems, giant plasma membrane vesicles (GPMVs) constitute an intermediate model between live cells and fully artificial structures. Certain applications, however, require planar membrane surfaces. Here, we report a new approach for creating supported plasma membrane bilayers (SPMBs) by bursting cell-derived GPMVs using ultrasound within a microfluidic device. We show that the mobility of outer leaflet molecules is preserved in SPMBs, suggesting that they are accessible on the surface of the bilayers. Such model membrane systems are potentially useful in many applications requiring detailed characterization of plasma membrane dynamics.
模型膜系统是在简化环境中研究生物过程以揭示潜在物理化学原理的重要工具。作为细胞衍生的膜系统，巨型质膜囊泡（GPMV）构成了活细胞与完全人工结构之间的中间模型。然而，某些应用需要平面膜表面。在此，我们报告了一种通过在微流控装置中使用超声使细胞衍生的GPMV破裂来创建支持的质膜双层（SPMB）的新方法。我们表明，外叶分子的流动性在SPMB中得以保留，这表明它们在双层表面是可及的。这种模型膜系统在许多需要详细表征质膜动力学的应用中可能很有用。
