Van Dijck Christophe, Cuylaerts Vicky, Sollie Piet, Spychala Anna, De Baetselier Irith, Laumen Jolein, Crucitti Tania, Kenyon Chris
Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Antwerp, 2000, Belgium.
Pharmacy Sollie, Antwerp, 2000, Belgium.
F1000Res. 2019 Sep 11;8:1620. doi: 10.12688/f1000research.20399.2. eCollection 2019.
: The oropharynx plays a major role in the development and spread of antimicrobial resistant among men who have sex with men. Trials are currently assessing the efficacy of bactericidal mouthwashes as possible therapeutic or preventive options against these pharyngeal gonococcal infections. Controlled clinical trials require the use of a placebo mouthwash without anti-gonococcal activity. So far, no such mouthwash has been described. We describe the development of a mouthwash for this purpose. : The anti-gonococcal activity of Corsodyl®, Listerine Cool Mint®, Biotene®, phosphate buffered saline and six in-house placebo mouthwashes was evaluated. Three gonococcal isolates from patients with pharyngeal infection were exposed to the mouthwashes for a duration ranging from 30 seconds to 60 minutes. Isolates were then plated onto blood agar (5% horse blood) and incubated for 24 hours (5-7% CO , 35 ± 2°C). Growth of was scored on a five-point scale (0 to 4). All experiments were conducted in duplicate. : Corsodyl® and Listerine Cool Mint® were bactericidal to all isolates. For the other mouthwashes, the median growth score after 60 minutes of exposure was 4 (interquartile range 4-4) for phosphate buffered saline; 1 (interquartile range 1-3) for Biotene®; and ranged between 0 and 2 for the in-house composed mouthwashes. An in-house composed mouthwash (Placebo 6) performed best, with a growth score of 2 (interquartile range 2-3). : All of the evaluated potential placebo mouthwashes were bacteriostatic after gonococcal exposure of 30 to 60 minutes. In-house composed Placebo 6 showed less inhibition on gonococcal growth than Biotene® and the other in-house placebos and demonstrates, in our opinion, a good trade-off between anti-gonococcal properties and taste.
口咽在男男性行为者中抗菌药物耐药性的产生和传播中起主要作用。目前正在进行试验，评估杀菌漱口水作为针对这些咽部淋球菌感染的可能治疗或预防选择的疗效。对照临床试验需要使用无抗淋球菌活性的安慰剂漱口水。到目前为止，尚未描述过此类漱口水。我们描述了一种为此目的开发的漱口水。：评估了含漱液、劲凉薄荷味漱口水、保丽净漱口水、磷酸盐缓冲盐水和六种自制安慰剂漱口水的抗淋球菌活性。将来自咽部感染患者的三株淋球菌分离株暴露于漱口水30秒至60分钟。然后将分离株接种到血琼脂（5%马血）上，在（5-7%二氧化碳，35±2°C）条件下孵育24小时。根据五分制（0至4）对生长情况进行评分。所有实验均重复进行。：含漱液和劲凉薄荷味漱口水对所有分离株均有杀菌作用。对于其他漱口水，暴露60分钟后的生长评分中位数，磷酸盐缓冲盐水为4（四分位间距4-4）；保丽净漱口水为1（四分位间距1-3）；自制漱口水的评分在0至2之间。一种自制漱口水（安慰剂6）表现最佳，生长评分为2（四分位间距2-3）。：所有评估的潜在安慰剂漱口水在淋球菌暴露30至60分钟后均具有抑菌作用。自制的安慰剂6对淋球菌生长的抑制作用小于保丽净漱口水和其他自制安慰剂，并且在我们看来，它在抗淋球菌特性和味道之间实现了良好的平衡。
