Chow Eric Pf, Howden Benjamin P, Walker Sandra, Lee David, Bradshaw Catriona S, Chen Marcus Y, Snow Anthony, Cook Stuart, Fehler Glenda, Fairley Christopher K
Melbourne Sexual Health Centre, Alfred Health, Melbourne, Victoria, Australia.
Faculty of Medicine, Nursing and Health Sciences, Central Clinical School, Monash University, Melbourne, Victoria, Australia.
Sex Transm Infect. 2017 Mar;93(2):88-93. doi: 10.1136/sextrans-2016-052753. Epub 2016 Dec 20.
Gonorrhoea is increasing among men who have sex with men (MSM). We aimed to determine whether Listerine, a commercial mouthwash product, has an inhibitory effect against in a randomised controlled trial (RCT) and an in vitro study, and therefore may be a potentially useful agent for gonorrhoea control.
In vitro: a suspension of ∼10 colony forming units per mL (CFU/mL) of was added to a serial of dilutions (up to 1:32) of alcohol-containing Listerine mouthwashes (Cool Mint and Total Care) for 1 min. A 10 µL aliquot was spread over the surface of a gonococcal agar plate and the number of colonies present at each dilution was calculated. The phosphate buffered saline (PBS) was used as a control. RCT: we recruited MSM with pharyngeal gonorrhoea who returned for treatment at the Melbourne Sexual Health Centre between May 2015 and February 2016. Untreated men were randomised to rinse and gargle either Listerine Cool Mint or saline for 1 min. Pharyngeal swabs were taken before and after rinsing and gargling for culture of . The analysis included only men who were culture positive for before using the allocated solution on the day of recruitment.
In vitro: Listerine mouthwashes at dilutions of up to 1:4 for 1 min resulted in significant reduction of total counts but PBS has no inhibitory effect against . RCT: a total of 196 MSM were recruited, 58 (30%) were culture positive before using the solution. After gargling the allocated solution, men in the Listerine group were significantly less likely to be culture positive on the pharyngeal surface (52%) compared with men in the saline group (84%) (p=0.013).
This data suggest Listerine, significantly reduces the amount of on the pharyngeal surface. With daily use it may increase gonococcal clearance and have important implications for prevention strategies.
ACTRN12615000716561.
男男性行为者（MSM）中的淋病发病率正在上升。我们旨在通过一项随机对照试验（RCT）和一项体外研究，确定市售漱口水产品李斯德林是否对淋病奈瑟菌有抑制作用，因此可能是一种控制淋病的潜在有用药物。
体外实验：将每毫升约10个菌落形成单位（CFU/mL）的淋病奈瑟菌悬液加入一系列稀释（最高1:32）的含酒精的李斯德林漱口水（劲爽薄荷味和全效护理型）中1分钟。取10微升等分试样铺在淋病奈瑟菌琼脂平板表面，计算每个稀释度下的淋病奈瑟菌菌落数。使用磷酸盐缓冲盐水（PBS）作为对照。RCT：我们招募了2015年5月至2016年2月期间在墨尔本性健康中心复诊治疗的咽部淋病男性患者。未治疗的男性被随机分为用劲爽薄荷味李斯德林或盐水漱口1分钟。在漱口前后采集咽部拭子用于淋病奈瑟菌培养。分析仅包括在招募当天使用分配溶液前淋病奈瑟菌培养呈阳性的男性。
体外实验：稀释至1:4的李斯德林漱口水作用1分钟可使淋病奈瑟菌总数显著减少，但PBS对淋病奈瑟菌无抑制作用。RCT：共招募了196名MSM，58名（30%）在使用溶液前培养呈阳性。漱口后，李斯德林组咽部表面培养呈阳性的男性比例（52%）显著低于盐水组（84%）（p = 0.013）。
这些数据表明，李斯德林可显著减少咽部表面的淋病奈瑟菌数量。每日使用可能会增加淋病奈瑟菌清除率，并对预防策略具有重要意义。
ACTRN12615000716561。
