School of Medicine and Dentistry, Santiago de Compostela University, Spain.
J Dent. 2010 Jul;38(7):541-6. doi: 10.1016/j.jdent.2010.03.012. Epub 2010 Apr 7.
To analyse the influence of intrinsic and extrinsic factors on the in vivo antimicrobial activity of a chlorhexidine (CHX) digluconate mouthwash on the salivary flora up to 7h after its application, using epifluorescence microscopy.
Ten volunteers performed the following mouthwashes: 0.12% CHX (10ml/30s, 15ml/30s and 10ml/1min); 0.2% CHX (10ml/30s, 15ml/30s and 10ml/1min); 0.2% CHX (10ml/30s) plus different daily activities (eating, drinking, chewing or smoking).
On comparing 0.12% CHX (10ml versus 15ml), the greatest differences in bacterial viability were detected at 1h and 3h. On comparing 0.12% CHX (30s versus 1min) the greatest differences in viability were detected at 1h, 3h, and 5h; and with 0.2% CHX (30s versus 1min), at 5h and 7h. On comparing 0.12% CHX (15ml) versus 0.2% CHX (10ml) and 0.12% CHX (1min) versus 0.2% CHX (30s), the percentage of viable bacteria was higher with the 0.12% concentration. On comparing 0.2% CHX versus 0.2% CHX plus daily activities, the higher differences were detected after eating and chewing, followed by drinking.
An increase in the volume of 0.12% or 0.2% CHX mouthwashes does not affect the duration of antimicrobial activity in saliva, whereas increasing the duration produces a marked increase in substantivity. Substantivity was greater with 0.2% CHX than 0.12% CHX. Eating, chewing or drinking significantly reduces the 0.2% CHX substantivity in saliva.
使用荧光显微镜分析内在和外在因素对氯己定（CHX）葡萄糖酸盐漱口水在应用后 7 小时内唾液菌群的体内抗菌活性的影响。
10 名志愿者进行以下漱口：0.12% CHX（10ml/30s，15ml/30s 和 10ml/1min）；0.2% CHX（10ml/30s，15ml/30s 和 10ml/1min）；0.2% CHX（10ml/30s）加不同的日常活动（进食、饮水、咀嚼或吸烟）。
比较 0.12% CHX（10ml 与 15ml）时，在 1 小时和 3 小时检测到最大的细菌活力差异。比较 0.12% CHX（30s 与 1min）时，在 1 小时、3 小时和 5 小时检测到最大的活力差异；0.2% CHX（30s 与 1min）时，在 5 小时和 7 小时检测到最大的活力差异。比较 0.12% CHX（15ml）与 0.2% CHX（10ml）和 0.12% CHX（1min）与 0.2% CHX（30s）时，0.12%浓度的活菌百分比更高。比较 0.2% CHX 与 0.2% CHX 加日常活动时，进食和咀嚼后差异较大，其次是饮水。
增加 0.12%或 0.2% CHX 漱口水的体积不会影响唾液中抗菌活性的持续时间，而增加持续时间会显著增加持续性。0.2% CHX 的持续性大于 0.12% CHX。进食、咀嚼或饮水会显著降低唾液中 0.2% CHX 的持续性。
