Analytical Chemistry Department, "Iuliu Hațieganu" University of Medicine and Pharmacy, 4, Louis Pasteur St., 400349, Cluj-Napoca, Romania.
Faculty of Biology and Geology, Electron Microscopy Center, "Babes-Bolyai" University, 5-7 Clinicilor St., 400006, Cluj-Napoca, Romania; National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat St., 400293, Cluj-Napoca, Romania.
Biosens Bioelectron. 2020 May 1;155:112098. doi: 10.1016/j.bios.2020.112098. Epub 2020 Feb 13.
A highly selective and sensitive molecularly imprinted polymer (MIP)-based electrochemical sensor was fabricated for the determination of azithromycin, a broad-spectrum macrolide antibiotic, from various biological samples (urine, tears, plasma). The reversible boronate ester bond-mediated, thin (~75 nm) MIP-based biomimetic recognition layer was electrodeposited in non-aqueous media onto the surface of a glassy carbon electrode (GCE). The surface morphology and the analytical performances of the developed sensor were assessed by scanning electron (SEM) and atomic force microscopy (AFM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). By employing an indirect electrochemical detection in the presence of 10 mM ferro/ferricyanide as redox probe, the sensor exhibited a very wide dynamic range (13.33 nM-66.67 μM), with an estimated detection limit in the subnanomolar range (0.85 nM azithromycin). The simple to construct sensor demonstrates reusability and good shelf-life, exhibiting remarkable selectivity over a wide number of structurally related and non-related antibiotics, commonly associated drugs and endogenous compounds.
一种高选择性和高灵敏度的基于分子印迹聚合物（MIP）的电化学传感器被制备出来，用于从各种生物样品（尿液、眼泪、血浆）中测定阿奇霉素，一种广谱的大环内酯类抗生素。通过可逆硼酸酯键介导，将厚度约为 75nm 的基于 MIP 的仿生识别层在非水介质中电沉积到玻碳电极（GCE）的表面上。通过扫描电子显微镜（SEM）和原子力显微镜（AFM）、电化学阻抗谱（EIS）和循环伏安法（CV）评估了所开发传感器的表面形貌和分析性能。通过在存在 10mM 铁氰化铁/亚铁作为氧化还原探针的情况下进行间接电化学检测，传感器表现出非常宽的动态范围（13.33nM-66.67μM），检测下限估计在纳摩尔范围内（0.85nM 阿奇霉素）。该传感器易于构建，具有良好的可重复使用性和保质期，对大量结构相关和非相关抗生素、常见药物和内源性化合物表现出显著的选择性。
