Department of Clinical Laboratory, Nanning Second People's Hospital, the Third Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
Department of Clinical Laboratory, Guangxi Hospital Of Traditional Chinese Medicine, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi, China.
J Clin Lab Anal. 2021 Feb;35(2):e23621. doi: 10.1002/jcla.23621. Epub 2020 Oct 15.
Through the detection of circular RNA (circRNA) using expression profiling chips, we searched for circRNAs related to acute myocardial infarction (AMI) and explored their relationship and possible mechanisms with AMI.
The study subjects included 3 AMI patients and 3 controls, and circRNA expression profiling analysis was performed using a microarray gene chip to identify circRNAs with large differences in expression between groups and to construct a circRNA-miRNA network.
Compared with the control group, there were 650 differentially expressed circRNAs found in AMI patients (P < .05, fold change > 2), including 535 up-regulated circRNAs, such as hsa_circ_0050908, hsa_circRNA4010-22, hsa_circ_0081241, hsa_circ_0010551, hsa_circRNA4010-20, hsa_circRNA14702, hsa_circ_0115392, has_circRNA1825-44, has_circRNA8493-7, and hsa_circ_0025097. Furthermore, there were 115 down-regulated circRNAs, such as hsa_circ_0066439, hsa_circ_0054211, hsa_circ_0095920, hsa_circ_0122984, hsa_circ_0113067, hsa_circ_0039155, hsa_circRNA4014-45, hsa_circ_0122979, hsa_circ_0059665, and hsa_circ_0009319. The circRNAs hsa_circ_0066439, hsa_circ_0081241, and hsa_circ_0122984 can regulate multiple signal pathways to participate in the AMI process through hsa-miR-1254, hsa-miR-328-5p, and other miRNAs. In addition, the expression of circRNA-miRNA in peripheral blood is related to the network. Differentially expressed circRNAs are involved in chromatin organization, chromatin-modifying enzymes, signal transduction, lysine degradation, the mitogen-activated protein kinase (MAPK) signaling pathway, focal adhesion, and a variety of other pathways, such as myocardial infarction, coronary heart disease, hypertension, and other diseases. The gene ontology analysis results show that molecular function mainly involves binding and molecular structural activity, whereas the biological process mainly involves a single biological process, a cellular component for organization, and a cellular process, and the cellular component mainly involves a protein complex, an extracellular matrix, and a membrane.
circRNA and microRNA interact to participate in the development of AMI. circRNA may be involved in the pathogenesis of AMI.
通过表达谱芯片检测环状 RNA（circRNA），寻找与急性心肌梗死（AMI）相关的 circRNA，并探讨其与 AMI 的关系及其可能的机制。
研究对象包括 3 例 AMI 患者和 3 例对照，采用微阵列基因芯片进行 circRNA 表达谱分析，筛选组间差异表达明显的 circRNA，并构建 circRNA-miRNA 网络。
与对照组相比，AMI 患者中发现有 650 个差异表达的 circRNA（P<.05，倍数变化>2），其中包括 535 个上调的 circRNA，如 hsa_circ_0050908、hsa_circRNA4010-22、hsa_circ_0081241、hsa_circ_0010551、hsa_circRNA4010-20、hsa_circRNA14702、hsa_circ_0115392、has_circRNA1825-44、has_circRNA8493-7 和 hsa_circ_0025097。此外，还有 115 个下调的 circRNA，如 hsa_circ_0066439、hsa_circ_0054211、hsa_circ_0095920、hsa_circ_0122984、hsa_circ_0113067、hsa_circ_0039155、hsa_circRNA4014-45、hsa_circ_0122979、hsa_circ_0059665 和 hsa_circ_0009319。circRNAs hsa_circ_0066439、hsa_circ_0081241 和 hsa_circ_0122984 可通过 hsa-miR-1254、hsa-miR-328-5p 等 miRNA 调节多个信号通路，参与 AMI 进程。此外，外周血中 circRNA-miRNA 的表达与网络有关。差异表达的 circRNAs 参与染色质组织、染色质修饰酶、信号转导、赖氨酸降解、丝裂原激活蛋白激酶（MAPK）信号通路、黏附、多种其他通路，如心肌梗死、冠心病、高血压等疾病。基因本体分析结果显示，分子功能主要涉及结合和分子结构活性，而生物过程主要涉及单一生物过程、组织、细胞过程，细胞成分主要涉及蛋白质复合物、细胞外基质和膜。
circRNA 与 microRNA 相互作用参与 AMI 的发生发展。circRNA 可能参与 AMI 的发病机制。
