van der Laan Kiran J, Morita Aryan, Perona-Martinez Felipe P, Schirhagl Romana
Department of Biomedical Engineering, University Medical Center Groningen, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
Department of Dental Biomedical Sciences, Faculty of Dentistry, Universitas Gadjah Mada, Jl Denta 1, Yogyakarta 55281, Indonesia.
Nanomaterials (Basel). 2020 Feb 20;10(2):372. doi: 10.3390/nano10020372.
Fluorescent nanodiamonds (FNDs) are proposed to be used as free radical biosensors, as they function as magnetic sensors, changing their optical properties depending on their magnetic surroundings. Free radicals are produced during natural cell metabolism, but when the natural balance is disturbed, they are also associated with diseases and aging. Sensitive methods to detect free radicals are challenging, due to their high reactivity and transiency, providing the need for new biosensors such as FNDs. Here we have studied in detail the stress response of an aging model system, yeast cells, upon FND internalization to assess whether one can safely use this biosensor in the desired model. This was done by measuring metabolic activity, the activity of genes involved in different steps and the locations of the oxidative stress defense systems and general free radical activity. Only minimal, transient FND-related stress effects were observed, highlighting excellent biocompatibility in the long term. This is a crucial milestone towards the applicability of FNDs as biosensors in free radical research.
荧光纳米金刚石（FNDs）被提议用作自由基生物传感器，因为它们可作为磁传感器，其光学特性会根据周围磁场环境而改变。自由基在细胞自然代谢过程中产生，但当自然平衡受到干扰时，它们也与疾病和衰老相关。由于自由基具有高反应活性和短暂性，检测自由基的灵敏方法具有挑战性，这就需要像FNDs这样的新型生物传感器。在此，我们详细研究了衰老模型系统酵母细胞在摄取FNDs后的应激反应，以评估是否能够在所需模型中安全使用这种生物传感器。这是通过测量代谢活性、参与不同步骤的基因活性、氧化应激防御系统的位置以及一般自由基活性来完成的。仅观察到与FNDs相关的最小、短暂的应激效应，突出了其长期优异的生物相容性。这是FNDs作为自由基研究中的生物传感器实现应用的关键里程碑。
