Sarkar Adrisha, Jones Zachary R, Parashar Madhur, Druga Emanuel, Akkiraju Amala, Conti Sophie, Krishnamoorthi Pranav, Nachuri Srisai, Aman Parker, Hashemi Mohammad, Nunn Nicholas, Torelli Marco D, Gilbert Benjamin, Wilson Kevin R, Shenderova Olga A, Tanjore Deepti, Ajoy Ashok
Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
Chemical Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Sci Adv. 2024 Dec 13;10(50):eadp4033. doi: 10.1126/sciadv.adp4033. Epub 2024 Dec 11.
A method is presented for high-precision chemical detection that integrates quantum sensing with droplet microfluidics. Using nanodiamonds (ND) with fluorescent nitrogen-vacancy (NV) centers as quantum sensors, rapidly flowing microdroplets containing analyte molecules are analyzed. A noise-suppressed mode of optically detected magnetic resonance is enabled by pairing controllable flow with microwave control of NV electronic spins, to detect analyte-induced signals of a few hundredths of a percent of the ND fluorescence. Using this method, paramagnetic ions in droplets are detected with low limit-of-detection using small analyte volumes, with exceptional measurement stability over >10 s. In addition, these droplets are used as microconfinement chambers by co-encapsulating ND quantum sensors with various analytes such as single cells, suggesting wide-ranging applications including single-cell metabolomics and real-time intracellular measurements from bioreactors. Important advances are enabled by this work, including portable chemical testing devices, amplification-free chemical assays, and chemical imaging tools for probing reactions within microenvironments.
本文介绍了一种将量子传感与微滴微流控技术相结合的高精度化学检测方法。使用具有荧光氮空位（NV）中心的纳米金刚石（ND）作为量子传感器，对含有分析物分子的快速流动微滴进行分析。通过将可控流动与NV电子自旋的微波控制相结合，实现了一种噪声抑制模式的光探测磁共振，以检测占ND荧光百分之几的分析物诱导信号。使用该方法，可在小分析物体积下以低检测限检测微滴中的顺磁性离子，在超过10秒的时间内具有出色的测量稳定性。此外，通过将ND量子传感器与各种分析物（如单细胞）共包封，这些微滴被用作微限制室，这表明其具有广泛的应用，包括单细胞代谢组学和生物反应器的实时细胞内测量。这项工作带来了重要进展，包括便携式化学检测设备、无扩增化学分析方法以及用于探测微环境内反应的化学成像工具。
