Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada.
Institute of Chemistry and The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem, Israel.
J Periodontal Res. 2020 Aug;55(4):503-510. doi: 10.1111/jre.12737. Epub 2020 Feb 24.
The aim of this study was to investigate whether a peptide-based coating can prevent the adhesion of Porphyromonas gingivalis, a key human pathogen associated with periodontitis and peri-implantitis.
Nonsurgical and surgical interventions have been used for the treatment of peri-implantitis; however, the effectiveness of these approaches is usually unsatisfactory. The main reason is that dental plaque on the surface of the implant is difficult to remove due to its rough surface and thread design. Recently, a peptide-based coating for implant surfaces that can reject the adhesion of Escherichia coli and improve the attachment of host cells was developed.
A salivary pellicle was created on the surfaces of peptide-coated bare discs and verified with anti-human immunoglobulin G, A and M, and anti-fibrinogen. Early colonizers, Veillonella parvula and Streptococcus sobrinus, and the later colonizer, Porphyromonas gingivalis, were labelled with green and red fluorescent dyes, respectively, and seeded on the discs. Bacterial attachment was semi-quantified by fluorescence intensity.
The salivary pellicle was evenly distributed on the discs, with or without the peptide coating, with an average thickness of 3.84 µm. A multi-species dental biofilm was created on the salivary pellicle. The peptide coating resulted in an approximate 25% reduction in the attachment of Veillonella parvula and Streptococcus sobrinus, and a 50% reduction in Porphyromonas gingivalis, when compared to control, uncoated implant discs.
The novel peptide-based coating can inhibit the attachment of Porphyromonas gingivalis. It may have the potential to impede the development of peri-implantitis.
本研究旨在探讨一种基于肽的涂层是否可以防止与牙周炎和种植体周围炎相关的关键人类病原体牙龈卟啉单胞菌的黏附。
已经使用非手术和手术干预措施来治疗种植体周围炎；然而，这些方法的效果通常并不令人满意。主要原因是由于种植体表面粗糙的表面和螺纹设计，表面的牙菌斑难以去除。最近，开发了一种用于种植体表面的基于肽的涂层，可以排斥大肠杆菌的黏附并改善宿主细胞的附着。
在肽涂层裸盘表面形成唾液膜，并通过抗人免疫球蛋白 G、A 和 M 以及抗纤维蛋白原进行验证。早期定植菌小韦荣球菌和变形链球菌，以及晚期定植菌牙龈卟啉单胞菌，分别用绿色和红色荧光染料标记，并接种在盘上。通过荧光强度对细菌附着进行半定量。
无论是否有肽涂层，唾液膜均匀分布在盘上，平均厚度为 3.84 µm。在唾液膜上形成了多物种牙科生物膜。与未涂层的对照种植体盘相比，肽涂层可使小韦荣球菌和变形链球菌的附着减少约 25%，牙龈卟啉单胞菌的附着减少 50%。
新型基于肽的涂层可以抑制牙龈卟啉单胞菌的附着。它可能有潜力阻止种植体周围炎的发展。
