Camargo Samira E A, Roy Tanaya, Carey Iv Patrick H, Fares Chaker, Ren Fan, Clark Arthur E, Esquivel-Upshaw Josephine F
Department of Restorative Dental Sciences, Division of Prosthodontics, University of Florida College of Dentistry, Gainesville, FL 32610, USA.
Department of Materials Science Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL 32611, USA.
J Funct Biomater. 2020 Jun 16;11(2):42. doi: 10.3390/jfb11020042.
Titanium nitride (TiN) and silicon carbide (SiC) adhesion properties to biofilm and the proliferation of human osteoblasts were studied. Quaternized titanium nitride (QTiN) was produced by converting the surface nitrogen on TiN to a positive charge through a quaternization process to further improve the antibacterial efficiency. The SiC required a nitridation within the plasma chamber of the surface layer before quaternization could be carried out to produce quaternized SiC (QSiC). The antimicrobial activity was evaluated on the reference strains of for 4 h by fluorescence microscopy using a live/dead viability kit. All the coatings exhibited a lower biofilm coverage compared to the uncoated samples (Ti-85.2%; TiN-24.22%; QTiN-11.4%; SiC-9.1%; QSiC-9.74%). Scanning Electron Microscope (SEM) images confirmed the reduction in bacteria on the SiC and TiN-coated groups. After 24 h of osteoblast cultivation on the samples, the cell adhesion was observed on all the coated and uncoated groups. Fluorescence images demonstrated that the osteoblast cells adhered and proliferated on the surfaces. TiN and SiC coatings can inhibit the attachment of and promote osteoblast adhesion on the titanium used for implants. These coatings may possess the ability to prevent the development of peri-implantitis and stimulate osteointegration.
研究了氮化钛（TiN）和碳化硅（SiC）与生物膜的粘附特性以及人成骨细胞的增殖情况。通过季铵化过程将TiN表面的氮转化为正电荷，从而制备出季铵化氮化钛（QTiN），以进一步提高抗菌效率。在进行季铵化以制备季铵化碳化硅（QSiC）之前，SiC需要在表面层的等离子体腔内进行氮化处理。使用活/死细胞活力试剂盒，通过荧光显微镜对参考菌株进行4小时的抗菌活性评估。与未涂层样品相比，所有涂层的生物膜覆盖率均较低（Ti-85.2%；TiN-24.22%；QTiN-11.4%；SiC-9.1%；QSiC-9.74%）。扫描电子显微镜（SEM）图像证实了SiC和TiN涂层组上细菌数量的减少。在样品上培养成骨细胞24小时后，在所有涂层和未涂层组上均观察到细胞粘附。荧光图像显示成骨细胞在表面粘附并增殖。TiN和SiC涂层可以抑制细菌附着，并促进成骨细胞在植入用钛上的粘附。这些涂层可能具有预防种植体周围炎发展和刺激骨整合的能力。
