Jusuf Nelva K, Putra Imam Budi, Dewi Nani Kumala
Department of Dermatology and Venereology, Faculty of Medicine, Universitas Sumatera Utara, Sumatera Utara, Indonesia.
Clin Cosmet Investig Dermatol. 2020 Jan 31;13:99-104. doi: 10.2147/CCID.S229743. eCollection 2020.
Passion fruit is used commercially for consumption and in beverages. This plant exhibits various pharmacological properties and possesses a complex phytochemistry. In recent years, this plant has been shown to have potential antimicrobial activity. The seeds contain a high amount of piceatannol, which exhibits an inhibitory effect on . Therefore, the seed extract of purple variant, , may have potential antibacterial activity against .
To evaluate the antibacterial activity of seeds extract on
Seed extract was prepared by maceration and dissolved in DMSO in multiple concentrations. was cultured in blood sheep 5% agar for 24 hours and the agar disc diffusion method was used to evaluate the inhibitory effect of each concentration (1.25%, 2.5%, 5%, 10%, 20%, and 40%), in comparison with clindamycin and erythromycin. Zones of inhibition at 24 hours were measured and documented, then analyzed to obtain the mean inhibition zone (MIZ). The Mann-Whitney test was conducted to compare the antibacterial activity of the extract, clindamycin and erythromycin. A serial dilution assay of five different concentrations (5%, 10%, 15%, 20%, and 25%) in brain-heart infusion broth was performed to determine the minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC).
After 24 hours, all concentrations of showed an inhibitory effect against , with MIZs for of each concentration of 1.25% 6 mm, 2.5% 6.83 mm, 5% 8.5 mm, 10% 10.08 mm, 20% 14 mm, and 40% 16 mm. Clindamycin revealed comparable antibacterial effect to 5% seed extract, with an MIZ of 8 mm. However, erythromycin was found to be superior to the seed extract, with a 22.67 mm MIZ (<0.05). The serial dilution assay revealed an MIC of 20% and MBC of 25%.
Seed extract of had good antibacterial activity against , and the effect increased with the concentration.
百香果被用于商业消费及制作饮品。这种植物具有多种药理特性且拥有复杂的植物化学组成。近年来，已表明这种植物具有潜在的抗菌活性。其种子含有大量的白皮杉醇，该物质对……具有抑制作用。因此，紫色变种百香果的种子提取物可能对……具有潜在抗菌活性。
评估百香果种子提取物对……的抗菌活性。
通过浸渍法制备种子提取物，并将其溶解于二甲基亚砜（DMSO）中配制成多种浓度。将……在含5%绵羊血的琼脂培养基中培养24小时，采用琼脂纸片扩散法评估各浓度（1.25%、2.5%、5%、10%、20%和40%）的抑制效果，并与克林霉素和红霉素作比较。测量并记录24小时后的抑菌圈，然后进行分析以获得平均抑菌圈（MIZ）。采用曼-惠特尼检验比较提取物、克林霉素和红霉素的抗菌活性。在脑心浸液肉汤中对五种不同浓度（5%、10%、15%、20%和25%）进行系列稀释试验，以确定最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。
24小时后，所有浓度的百香果种子提取物均对……表现出抑制作用，各浓度的平均抑菌圈分别为：1.25%为6毫米，2.5%为6.83毫米，5%为8.5毫米，10%为10.08毫米，20%为14毫米，40%为16毫米。克林霉素显示出与5%种子提取物相当的抗菌效果，平均抑菌圈为8毫米。然而，发现红霉素优于种子提取物，平均抑菌圈为22.67毫米（P<0.05）。系列稀释试验显示最低抑菌浓度为20%，最低杀菌浓度为25%。
百香果种子提取物对……具有良好的抗菌活性，且抗菌效果随浓度增加而增强。
