Liu Bihui, Li Ji, Yi Ruokun, Mu Jianfei, Zhou Xianrong, Zhao Xin
Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China.
Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing 400067, China.
Foods. 2019 Jan 19;8(1):36. doi: 10.3390/foods8010036.
is a traditional Chinese food that can be used as a beverage. In this study, three kinds of from different regions of China were selected to observe the preventive effects of extracted alkaloids on CCl₄-induced liver injuries. Animal experiments revealed that alkaloids extracted from decreased the serum AST (aspartate aminotransferase), ALT (alanine aminotransferase), and TBIL (total bilirubin) levels, enhanced SOD (superoxide dismutase) activity, and reduced MDA (malondialdehyde) level in the liver tissues of mice with liver injury. H&E observation confirmed that alkaloids from could alleviate CCl₄-induced injuries of liver tissues and inhibit the inflammatory effect on hepatocytes. Further qPCR experiments also demonstrated that alkaloids from upregulated the expression of IκB-α (inhibitor of NF-κB alpha), Cu/Zn-SOD (copper/zinc superoxide dismutase), Mn-SOD (manganese superoxide dismutase), and CAT (catalase) mRNA and downregulated TNF-α (tumor necrosis factor alpha) and NF-κB (nuclear factor kappa B) expression in the liver tissues of mice with liver injury. All three kinds of alkaloids from could prevent CCl₄-induced liver injuries by regulating the levels of oxidative stress and inflammation in mice, and the therapeutic effect was comparable to that of silymarin, the medicine commonly used in the treatment of liver diseases. In summary, alkaloids from contain bioactive substances with hepatic protective efficacy and possess potential application value in the field of functional food.
是一种可作为饮品的传统中式食品。在本研究中，选取了来自中国不同地区的三种[具体食品名称未给出]，以观察提取的生物碱对四氯化碳诱导的肝损伤的预防作用。动物实验表明，从[具体食品名称未给出]中提取的生物碱可降低肝损伤小鼠血清中的天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）和总胆红素（TBIL）水平，增强超氧化物歧化酶（SOD）活性，并降低肝组织中丙二醛（MDA）水平。苏木精-伊红（H&E）观察证实，来自[具体食品名称未给出]的生物碱可减轻四氯化碳诱导的肝组织损伤，并抑制对肝细胞的炎症作用。进一步的定量聚合酶链反应（qPCR）实验还表明？来自[具体食品名称未给出]的生物碱上调了肝损伤小鼠肝组织中IκB-α（核因子κBα抑制剂）、铜/锌超氧化物歧化酶（Cu/Zn-SOD）、锰超氧化物歧化酶（Mn-SOD）和过氧化氢酶（CAT）mRNA的表达，并下调肿瘤坏死因子α（TNF-α）和核因子κB（NF-κB）的表达。来自[具体食品名称未给出]的所有三种生物碱均可通过调节小鼠体内氧化应激和炎症水平来预防四氯化碳诱导的肝损伤，其治疗效果与治疗肝病常用药物水飞蓟素相当。综上所述，来自[具体食品名称未给出]的生物碱含有具有肝脏保护功效 的生物活性物质，在功能性食品领域具有潜在的应用价值。
原文中“three kinds of from different regions of China”和“three kinds of alkaloids from ”处有信息缺失，已按格式要求翻译，但这部分内容不完整可能影响对整体的理解。
