Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington 98195, United States.
Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045, United States.
J Nat Prod. 2020 Mar 27;83(3):738-743. doi: 10.1021/acs.jnatprod.9b01237. Epub 2020 Feb 27.
Bactobolin is a hybrid natural product with potent cytotoxic activity. Its production from was reported as part of a collaboration between the Greenberg and Clardy laboratories in 2010. The collaboration sparked a series of studies leading to the discovery of new analogues and associated structure-activity relationships, the identification of the bactobolin biosynthetic gene cluster and assembly of its unusual amino acid building block, the molecular target of and resistance to the antibiotic, and finally an X-ray crystal structure of the ribosome-bactobolin complex. Herein, we review the collaborations that led to our current understanding of the chemistry and biology of bactobolin.
杆菌肽是一种具有强大细胞毒性的混合天然产物。其生产菌 被报道是 2010 年格林伯格和克拉迪实验室合作的一部分。这次合作引发了一系列研究，发现了新的类似物和相关的结构-活性关系，鉴定了杆菌肽生物合成基因簇和其不寻常的氨基酸构建块的组装，确定了抗生素的分子靶标和耐药性，最终获得了核糖体-杆菌肽复合物的 X 射线晶体结构。本文综述了导致我们目前对杆菌肽化学和生物学理解的合作。
