Shanghai Key Lab of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, Tongji University, Shanghai 200092, China.
School of Materials Science and Engineering, Liaocheng University, Liaocheng 252059, China.
ACS Appl Mater Interfaces. 2020 Mar 18;12(11):12990-12997. doi: 10.1021/acsami.0c00101. Epub 2020 Mar 9.
Identifying biomolecules for disease diagnosis requires simple, accurate, and reliable analytical techniques. Multiple signal transduction pathways have promoted the development of various biological analysis systems. However, most systems are largely limited by a single mechanism or model analysis, which can easily lead to false-positive/negative results. Herein, we report a covalent organic framework (COF) () functionalized with a dye (fluorescein sodium) and design this hybrid material () to fabricate hydrogels for subsequent analysis with the indicator displacement assay (IDA) method. Selecting a suitable metal cation (Cr) for the preparation of hydrogels can reduce the background fluorescence, improve the detection sensitivity, and increase the corresponding sensing selectivity. The functions as an indicator in the IDA-in-COF system, and Cr is a receptor of the analyte (sialic acid (SA), a biomarker for ovarian cancer diagnosis). Based on the above studies, the integrative logic operations (AND + IMP) are further established, it helps in elucidating the design rules of the IDA-in-COF approach. This work represents the first effort in designing IDA-in-COF luminescent sensors with an On-Off-On mechanism to determine biomarkers and provides a new approach for developing hybrid COF luminescent materials as analysis platforms for human health monitoring.
用于疾病诊断的生物分子的识别需要简单、准确和可靠的分析技术。多种信号转导途径促进了各种生物分析系统的发展。然而，大多数系统在很大程度上受到单一机制或模型分析的限制，这很容易导致假阳性/阴性结果。在此，我们报告了一种用染料（荧光素钠）功能化的共价有机框架（COF）()，并设计了这种杂化材料（）来制备水凝胶，以便随后用指示剂置换分析（IDA）方法进行分析。选择合适的金属阳离子（Cr）来制备水凝胶可以降低背景荧光，提高检测灵敏度，并增加相应的传感选择性。在 IDA-in-COF 体系中，作为指示剂，Cr 是分析物（唾液酸（SA），一种用于卵巢癌诊断的生物标志物）的受体。基于上述研究，进一步建立了集成逻辑运算（AND + IMP），有助于阐明 IDA-in-COF 方法的设计规则。这项工作代表了首次设计具有开-关-开机制的 IDA-in-COF 荧光传感器以确定生物标志物的努力，并为开发混合 COF 荧光材料作为人类健康监测的分析平台提供了一种新方法。
