Advanced Technologies Application and Research Center, Hacettepe University, Ankara, Turkey.
Department of Chemistry, Aksaray University, Aksaray, Turkey.
Talanta. 2020 May 15;212:120778. doi: 10.1016/j.talanta.2020.120778. Epub 2020 Jan 23.
Tailor-made Escherichia coli (E. coli) receptors were created with microcontact imprinted technique and binding events of E. coli were carried out by a surface plasmon resonance (SPR) sensor in aqueous solution and in urine mimic in real time and label-free. N-methacryloyl-(l)-histidine methyl ester (MAH) was selected as a functional monomer to design tailor-made E. coli receptors on the polymeric film and during the formation of the polymeric film on a chip surface, Ag nanoparticles (AgNPs) were entrapped into the polymer mixture in order to lower the detection limit of biomimetic SPR based sensor. The polymeric film was characterized with atomic force microscopy (AFM), scanning electron microscopy (SEM), ellipsometer and contact angle measurements. Limit of detection (LOD) was found 0.57 CFU/mL and feasibility of the biomimetic sensor was investigated in urine mimic.
采用微接触印迹技术定制了大肠杆菌（E. coli）受体，通过表面等离子体共振（SPR）传感器在水溶液中和实时无标记的模拟尿液中进行了 E. coli 的结合事件。选择 N-丙烯酰基-（L）-组氨酸甲酯（MAH）作为功能单体，在聚合物薄膜上设计定制的大肠杆菌受体，在芯片表面形成聚合物薄膜的过程中，将银纳米粒子（AgNPs）掺入聚合物混合物中，以降低基于仿生 SPR 的传感器的检测限。通过原子力显微镜（AFM）、扫描电子显微镜（SEM）、椭圆偏振仪和接触角测量对聚合物薄膜进行了表征。检测限（LOD）为 0.57 CFU/mL，并且在模拟尿液中研究了仿生传感器的可行性。
