Yadav Kanta, Bajaj Rajesh Kumar, Mandal Surajit, Mann Bimlesh
1Dairy Chemistry Division, ICAR- National Dairy Research Institute, Karnal, Haryana 132001 India.
2Dairy Microbiology Division, ICAR- National Dairy Research Institute, Karnal, Haryana 132001 India.
J Food Sci Technol. 2020 Feb;57(2):426-434. doi: 10.1007/s13197-019-04070-4. Epub 2019 Sep 5.
Grape seed extract (GSE) contain phenolic compounds that decrease the proclivity to various chronic diseases such as several types of cancer and cardiovascular diseases. The objective of the present study was to investigate the encapsulation of GSE polyphenols and their characterization. For this study, whey protein concentrate (WPC), maltodextrin (MD) and gum arabic (GA) were evaluated as encapsulating materials. For the preparation of stable microcapsules different WPC:MD/GA (5:0, 4:1, 3:2 and 0:5) ratios were assessed using ultrasonication for different time periods (20-40 min) followed by freeze drying. Encapsulation efficiency, antioxidant activity, particle size, surface morphology and release mechanism were determined. The GSE microcapsules coated with WPC:MD/GA ratio of 4:1 and 3:2 with core to coat ratio of 1:5 and prepared by sonication for 30 min were found to have highest encapsulation efficiency (87.90-91.13%) and the smallest particle size with maximum retention of antioxidant activity. Under optimized conditions, the low level release (43-49%) of phenolic compounds resulted under simulated gastric condition and significantly ( < 0.05) increased (88-92%) under simulated intestinal condition. Thus the results indicated blending of MD or GA with WPC improved the microencapsulation of GSE.
葡萄籽提取物(GSE)含有酚类化合物,可降低患多种慢性疾病的倾向,如某些类型的癌症和心血管疾病。本研究的目的是研究GSE多酚的包封及其特性。在本研究中,评估了乳清蛋白浓缩物(WPC)、麦芽糊精(MD)和阿拉伯胶(GA)作为包封材料。为了制备稳定的微胶囊,使用超声处理不同时间段(20 - 40分钟),然后冷冻干燥,评估了不同的WPC:MD/GA(5:0、4:1、3:2和0:5)比例。测定了包封效率、抗氧化活性、粒径、表面形态和释放机制。发现以4:1和3:2的WPC:MD/GA比例包被、核壳比为1:5并通过超声处理30分钟制备的GSE微胶囊具有最高的包封效率(87.90 - 91.13%)和最小的粒径,同时最大程度地保留了抗氧化活性。在优化条件下,模拟胃条件下酚类化合物的低水平释放(43 - 49%),而在模拟肠条件下显著增加(<0.05)(88 - 92%)。因此,结果表明MD或GA与WPC混合可改善GSE的微胶囊化。