Deist Melissa S, Gallardo Rodrigo A, Dekkers Jack C M, Zhou Huaijun, Lamont Susan J
Department of Animal Science, Iowa State University, Ames, IA, United States.
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, Davis, CA, United States.
Front Genet. 2020 Feb 4;11:11. doi: 10.3389/fgene.2020.00011. eCollection 2020.
Disease has large negative impacts on poultry production. A more comprehensive understanding of host-pathogen interaction can lead to new and improved strategies to maintain health. In particular, host genetic factors can lead to a more effective response to pathogens, hereafter termed resistance. Fayoumi and Leghorn chicken lines have demonstrated relative resistance and susceptibility, respectively, to the Newcastle disease virus (NDV) vaccine strain and many other pathogens. This biological model was used to better understand the host response to a vaccine strain of NDV across three tissues and time points, using RNA-seq. Analyzing the Harderian gland, trachea, and lung tissues together using weighted gene co-expression network analysis (WGCNA) identified important genes that were co-expressed and associated with parameters including: genetic line, days post-infection (dpi), challenge status, sex, and tissue. Pathways and driver genes, such as , , and , associated with challenge status, dpi, and genetic line were of particular interest as candidates for disease resistance. Overall, by jointly analyzing the three tissues, this study identified genes and gene networks that led to a more comprehensive understanding of the whole animal response to lentogenic NDV than that obtained by analyzing the tissues individually.
疾病对家禽生产有巨大的负面影响。对宿主 - 病原体相互作用有更全面的了解可以带来新的和改进的保持健康的策略。特别是，宿主遗传因素可以导致对病原体产生更有效的反应，以下称为抗性。法尤米鸡和来航鸡品系分别对新城疫病毒（NDV）疫苗株和许多其他病原体表现出相对抗性和易感性。利用RNA测序技术，这个生物学模型被用于更好地了解宿主在三个组织和时间点对NDV疫苗株的反应。使用加权基因共表达网络分析（WGCNA）对哈氏腺、气管和肺组织进行综合分析，确定了共表达且与包括遗传品系、感染后天数（dpi）、攻毒状态、性别和组织等参数相关的重要基因。与攻毒状态、dpi和遗传品系相关的通路和驱动基因，如 、 和 ，作为抗病候选基因特别受关注。总体而言，通过对这三个组织进行联合分析，本研究确定的基因和基因网络比单独分析组织能更全面地了解整个动物对缓发型NDV的反应。
