Aspergillus Unit, Pasteur Institut, Paris, France.
Infectious Diseases in Global Health Program, Centre for Translational Biology, McGill University Health Centre, Montréal, QC, Canada.
Front Cell Infect Microbiol. 2020 Feb 6;10:28. doi: 10.3389/fcimb.2020.00028. eCollection 2020.
Deacetylation of chitin by chitin deacetylases (Cda) results in the formation of chitosan. Chitosan, a polymer of β1,4 linked glucosamine, plays multiple roles in the function of the fungal cell wall, including virulence and evasion of host immune responses. In this study, the roles of chitosan and putative s in cell wall structure and virulence of were investigated. Low levels of chitosan were found in the conidial and cell wall of . Seven putative genes were identified, disrupted and the phenotype of the single mutants and the septuple mutants were investigated. No alterations in fungal cell wall chitosan levels, changes in fungal growth or alterations in virulence were detected in the single or septuple Δ mutant strains. Collectively, these results suggest that chitosan is a minority component of the cell wall, and that the seven candidate Cda proteins do not play major roles in fungal cell wall synthesis or virulence. However, Cda2 is involved in conidiation, suggesting that this enzyme may play a role in N-acetyl-glucosamine metabolism.
壳聚糖由壳聚糖脱乙酰酶（Cda）脱乙酰化形成。壳聚糖是β1,4 连接的葡萄糖胺的聚合物，在真菌细胞壁的功能中发挥多种作用，包括毒力和逃避宿主免疫反应。在这项研究中，研究了壳聚糖和推定的 s 在 的细胞壁结构和毒力中的作用。在分生孢子和细胞壁中发现 的壳聚糖含量较低。鉴定了七个推定的 基因，对其进行了破坏，并研究了单突变体和七重突变体的表型。在单突变体或七重突变体菌株中未检测到真菌细胞壁壳聚糖水平的变化、真菌生长的变化或毒力的改变。总的来说，这些结果表明壳聚糖是 的细胞壁的少数成分，并且这七个候选 Cda 蛋白在真菌细胞壁合成或毒力中不起主要作用。然而，Cda2 参与分生孢子形成，表明该酶可能在 N-乙酰葡萄糖胺代谢中发挥作用。
