Cancer Biology, CSIR-Centre for Cellular and Molecular Biology, (CCMB), Uppal Road, Hyderabad, 500007, India.
Bioinformatics, CSIR-Centre for Cellular and Molecular Biology, (CCMB), Uppal Road, Hyderabad, 500007, India.
Database (Oxford). 2020 Jan 1;2020. doi: 10.1093/database/baz151.
Acute lymphoblastic leukemia (ALL) is one of the most common hematological malignancies in children. Recent studies suggest the involvement of multiple microRNAs in the tumorigenesis of various leukemias. However, until now, no comprehensive database exists for miRNAs and their cognate target genes involved specifically in ALL. Therefore, we developed 'LeukmiR' a dynamic database comprising in silico predicted microRNAs, and experimentally validated miRNAs along with the target genes they regulate in mouse and human. LeukmiR is a user-friendly platform with search strings for ALL-associated microRNAs, their sequences, description of target genes, their location on the chromosomes and the corresponding deregulated signaling pathways. For the user query, different search modules exist where either quick search can be carried out using any fuzzy term or by providing exact terms in specific modules. All entries for both human and mouse genomes can be retrieved through multiple options such as miRNA ID, their accession number, sequence, target genes, Ensemble-ID or Entrez-ID. User can also access miRNA: mRNA interaction networks in different signaling pathways, the genomic location of the targeted regions such as 3'UTR, 5'UTR and exons with their gene ontology and disease ontology information in both human and mouse systems. Herein, we also report 51 novel microRNAs which are not described earlier for ALL. Thus, LeukmiR database will be a valuable source of information for researchers to understand and investigate miRNAs and their targets with diagnostic and therapeutic potential in ALL. Database URL: http://tdb.ccmb.res.in/LeukmiR/.
急性淋巴细胞白血病（ALL）是儿童中最常见的血液系统恶性肿瘤之一。最近的研究表明，多种 microRNAs 参与了各种白血病的肿瘤发生。然而，到目前为止，还没有专门针对 ALL 的 microRNAs 及其同源靶基因的综合数据库。因此，我们开发了“LeukmiR”，这是一个动态数据库，包含计算机预测的 microRNAs 和在小鼠和人类中经过实验验证的 microRNAs 及其调控的靶基因。LeukmiR 是一个用户友好的平台，具有用于 ALL 相关 microRNAs、它们的序列、靶基因描述、它们在染色体上的位置以及对应的失调信号通路的搜索字符串。对于用户查询，存在不同的搜索模块，用户可以使用任何模糊术语进行快速搜索，也可以在特定模块中提供精确术语进行搜索。通过多种选项，如 miRNA ID、它们的访问号、序列、靶基因、Ensemble-ID 或 Entrez-ID，可以检索人类和小鼠基因组的所有条目。用户还可以访问不同信号通路中的 miRNA:mRNA 相互作用网络，靶向区域的基因组位置，如 3'UTR、5'UTR 和外显子，以及它们在人类和小鼠系统中的基因本体论和疾病本体论信息。在此，我们还报告了 51 种新的 microRNAs，它们以前没有被描述为 ALL。因此，LeukmiR 数据库将成为研究人员的宝贵信息来源，用于理解和研究 ALL 中具有诊断和治疗潜力的 microRNAs 及其靶标。数据库网址：http://tdb.ccmb.res.in/LeukmiR/。
