Dreher R
Immun Infekt. 1977 Feb;5(1):11-22.
ANA typing of RA patients according to the heavy- and light chains, complement fixing ability and immunofluorescent staining pattern was performed by the immunofluorescence technique and partially by qualitative immunoelectrophoresis. In the corresponding sera the immunoglobulins and complement components were quantiatively determined. Dependent on the ANA-immunoglobulin classes we found the following distribution: IgG-ANA; 40/93 (43%), IgM-ANA: 19/93 (20%), IgA-ANA: 2/93 (2%), and Ig-comb.-ANA:17/93 (18%). ANA only positive for polyspecific antimmunoglobulin serum: 15/93 (16%). After L-chain typing we found within a given ANA-Ig-class positivity for both L-chain subclasses (kappa and lambda): IgG-ANA: 19/40 (48%), IgM-ANA: 3/19 (16%), IgA-ANA:1/1 and Ig-comb.-ANA: 14/17 (82%). ANA cases within a given Ig-class only positive for the L-chain/type kappa:IgM-ANA:6/19 (32%), IgG-ANA: 9/40 (23%), Ig-comb.-ANA:2/12 (12%). ANA cases within a given Ig-class only positive for the L-chain type lambda: IgM-ANA: 7/19 (37%), IgG-ANA: 3/40 (8%), Ig-comb.-ANA:1/17 (6%) and IgA-ANA:1/1. In all cases of restricted ANA-positivity (only positive by use of polyspecific anti-immunoglobulin serum) no light chains were detected. In total we found approximatively 25% of complement fixing ANA, predominantly IgG-ANA (70%). Concerning the immunofluorescent nuclear staining we stated the following patterns: homogenous: 34/93 (36.6%), speckled: 29/93 (31.2%), mixed pattern : 22/93 (23.6%) peripheral: 5/93 (5.4%) and nucleolar: 3/93 (3.2%). The quantitative Ig-determination reveals a significant increase of IgG in ANA (single type) positive sera. IgG + IGM + IGA-ANA and IgG + IgM-ANA are found together with a significantly increased IgG- and IgM-serum levels, in the case of IgG + IgM-ANA we could demonstrate an additional relative decrease of the complement component C4. Sera with positive complement fixing ANA compared with non complement fixing ANA are characterized by an absolute increase of IgG and relative decrease of the complement component C4. No differences in serum-immunoglobulin- and -complement concentrations were registered dependent on ANA-immunofluorescent staining patterns.
采用免疫荧光技术并部分通过定性免疫电泳对类风湿关节炎（RA）患者进行抗核抗体（ANA）分型，依据重链和轻链、补体结合能力以及免疫荧光染色模式进行。对相应血清中的免疫球蛋白和补体成分进行定量测定。根据ANA免疫球蛋白类别，我们发现了以下分布情况：IgG - ANA：40/93（43%），IgM - ANA：19/93（20%），IgA - ANA：2/93（2%），以及Ig组合 - ANA：17/93（18%）。仅对多特异性抗免疫球蛋白血清呈阳性的ANA：15/93（16%）。轻链分型后，在给定的ANA - Ig类别阳性中，我们发现两种轻链亚类（κ和λ）均呈阳性：IgG - ANA：19/40（48%），IgM - ANA：3/19（16%），IgA - ANA：1/1，Ig组合 - ANA：14/17（82%）。在给定Ig类别中仅对κ轻链/型呈阳性的ANA病例：IgM - ANA：6/19（32%），IgG - ANA：9/40（23%），Ig组合 - ANA：2/12（12%）。在给定Ig类别中仅对λ轻链型呈阳性的ANA病例：IgM - ANA：7/19（37%），IgG - ANA：3/40（8%），Ig组合 - ANA：1/17（6%），IgA - ANA：1/1。在所有ANA阳性受限的病例（仅使用多特异性抗免疫球蛋白血清呈阳性）中，未检测到轻链。总体而言，我们发现约25%的ANA具有补体结合能力，主要为IgG - ANA（70%）。关于免疫荧光核染色，我们观察到以下模式：均匀型：34/93（36.6%），斑点型：29/93（31.
