Al-Awaida Wajdy J, Sharab Ahmad S, Al-Ameer Hamzeh J, Ayoub Nabil Y
1Department of Biology and Biotechnology, American University of Madaba, Madaba, Jordan.
2Department of Basic Sciences and Humanities, Faculty of Science, American University of Madaba (AUM), Amman, 11821 Jordan.
NPJ Microgravity. 2020 Feb 24;6:6. doi: 10.1038/s41526-020-0096-x. eCollection 2020.
Microgravity affects plant growth and content. A three-dimensional clinostat was used at 4 rotations/min to rotate the seeds of cultivar (Ammon) in three dimensions for 7 days, following which the antioxidant activities of ethanolic extracts were evaluated using both nitric oxide- and hydrogen peroxide-scavenging activities. The antidiabetic activities of ethanolic extracts were evaluated by measuring the concentration of plasma glucose, insulin, C peptide, and glycated hemoglobin (HbA1c); determining the number of β cells in the pancreatic islets; and performing the glucose tolerance test. Furthermore, the effects of the ethanolic extracts on the lipid profile and liver function were estimated. After rats were sacrificed, their pancreases were isolated and used for histopathological processing. The results indicated that the antioxidant potential and antioxidant metabolite content were significantly increased under microgravity conditions in comparison to those under normal gravity conditions. Rats treated with an extract of wheatgrass () germinated over a period of 6-10 days under microgravity (WGM) showed a significant reduction in the levels of serum glucose, HbA1C, urea, creatinine, aspartate aminotransferase and alanine aminotransferase, and insulin resistance compared to rats treated with an extract of wheatgrass germinated under gravity. Additionally, the total cholesterol and low-density lipoprotein cholesterol levels were significantly decreased. In contrast, high-density lipoprotein cholesterol, C-peptide, and insulin levels rose significantly after treatment with germinated under microgravity. WGM is a promising potential diabetic treatment without side effects with a low manufacturing cost.
微重力会影响植物生长和成分。使用三维回转器以每分钟4转的速度对品种（Ammon）的种子进行三维旋转处理7天，之后使用一氧化氮清除活性和过氧化氢清除活性来评估乙醇提取物的抗氧化活性。通过测量血浆葡萄糖、胰岛素、C肽和糖化血红蛋白（HbA1c）的浓度；确定胰岛中β细胞的数量；以及进行葡萄糖耐量试验来评估乙醇提取物的抗糖尿病活性。此外，还评估了乙醇提取物对血脂和肝功能的影响。大鼠处死后，分离其胰腺并用于组织病理学处理。结果表明，与正常重力条件下相比，微重力条件下抗氧化潜力和抗氧化代谢物含量显著增加。与用在重力条件下发芽的小麦草提取物处理的大鼠相比，用在微重力条件下6 - 10天发芽的小麦草（）提取物处理的大鼠血清葡萄糖、HbA1C、尿素、肌酐、天冬氨酸转氨酶和丙氨酸转氨酶水平以及胰岛素抵抗显著降低。此外，总胆固醇和低密度脂蛋白胆固醇水平显著降低。相比之下，用在微重力条件下发芽的处理后，高密度脂蛋白胆固醇、C肽和胰岛素水平显著升高。微重力条件下发芽的小麦草是一种有前景的潜在糖尿病治疗方法，无副作用且制造成本低。
