填补过敏原检测中的分析空白——实时 PCR 法检测食品中商业相关头足类和腹足类动物。
Filling Analytical Gaps in Allergen Detection─Real-Time PCR for the Detection of Commercially Relevant Cephalopods and Gastropods in Food.
机构信息
Department of Food Safety, German Federal Institute for Risk Assessment (BfR), P.O. Box 330013, D-14191 Berlin, Germany.
Technische Universität Berlin, Institute for Food Technology and Food Chemistry, Gustav-Meyer-Allee 25, 13355 Berlin, Germany.
出版信息
J Agric Food Chem. 2023 Aug 9;71(31):12029-12042. doi: 10.1021/acs.jafc.2c08966. Epub 2023 Jul 27.
Mollusks belong to the group of shellfish, which are considered to be among the elicitors of severe food allergies worldwide. In recent years, numerous PCR detection methods have been developed for other shellfish such as crustaceans. However, cephalopods and gastropods were not considered in the development of these shellfish detection systems. In this study, we have developed highly specific real-time PCR methods for the comprehensive detection of all commercially relevant cephalopod species and the gastropod families Helicidae, Buccinidae, and Muricidae in food matrices. In total, we cross-tested over 100 animal and plant species to show the specificity of our systems. The limit of detection (LOD) was set at 1 pg of cephalopod and gastropod DNA or 10 ppm (mg/kg) spiked in a vegetarian food product. The robustness of the protocol was confirmed by testing multiple parameters while cooking and autoclaving of samples ensured the practical applicability of the systems.
软体动物属于贝类，被认为是全球引发严重食物过敏的过敏原之一。近年来，已经开发出了许多针对其他贝类（如甲壳类动物）的 PCR 检测方法。然而，在这些贝类检测系统的开发中，头足类动物和腹足类动物并未被考虑在内。在这项研究中，我们开发了高度特异性的实时 PCR 方法，用于全面检测食品基质中的所有商业相关头足类动物物种以及腹足纲的 Helicidae、Buccinidae 和 Muricidae 科。总共有 100 多种动植物物种被交叉测试，以证明我们系统的特异性。检测限（LOD）设定为 1pg 的头足类和腹足类动物 DNA 或在素食食品产品中添加 10ppm（mg/kg）。通过测试烹饪和高压灭菌过程中的多个参数来确认该方案的稳健性，从而确保了这些系统的实际适用性。
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