西班牙拉里奥哈地区卫生工作者中某物种的流行情况（血清学、培养和聚合酶链反应）
spp. Prevalence (Serology, Culture, and PCR) in Sanitary Workers in La Rioja Spain.
作者信息
Portillo Aránzazu, Maggi Ricardo, Oteo José A, Bradley Julie, García-Álvarez Lara, San-Martín Montserrat, Roura Xavier, Breitschwerdt Edward
机构信息
Departamento de Enfermedades Infecciosas, Hospital U. San Pedro-Centro de Investigación Biomédica de La Rioja (CIBIR), 26006 Logroño, La Rioja, Spain.
Galaxy Diagnostics, Research Triangle Park, Morrisville, NC 27709, USA.
出版信息
Pathogens. 2020 Mar 4;9(3):189. doi: 10.3390/pathogens9030189.
spp. are increasingly implicated in association with a spectrum of zoonotic infectious diseases. One hundred sanitary workers in La Rioja, Spain completed a questionnaire and provided blood specimens for spp. serology and alpha-Proteobacteria growth medium (BAPGM) enrichment blood culture/PCR. Six immunofluorescence assays (IFA) were performed and aseptically obtained blood specimens were inoculated into liquid BAPGM and subcultured onto blood agar plates. DNA was amplified using conventional and real-time PCR assays. The spp., strain, or genotype was determined by DNA sequencing. seroreactivity was documented in 83.1% and bloodstream infection in 21.6% of participants. , subsp. genotypes I and III, and were identified. IFA seroreactivity and PCR positivity were not statistically associated with self-reported symptoms. Our results suggest that exposure to and non-clinical infection with spp. may occur more often than previously suspected in the La Rioja region.
某些物种越来越多地与一系列人畜共患传染病相关联。西班牙拉里奥哈的100名卫生工作者填写了一份问卷，并提供了血液样本用于某些物种的血清学检测以及α-变形杆菌生长培养基（BAPGM）富集血培养/聚合酶链反应（PCR）。进行了六项免疫荧光测定（IFA），并将无菌采集的血液样本接种到液体BAPGM中，然后转种到血琼脂平板上。使用传统和实时PCR测定法扩增DNA。通过DNA测序确定某些物种、菌株或基因型。83.1%的参与者记录有血清反应性，21.6%的参与者有血流感染。鉴定出了某些物种、亚种基因型I和III以及其他物种。IFA血清反应性和PCR阳性与自我报告的症状无统计学关联。我们的结果表明，在拉里奥哈地区，接触某些物种并发生非临床感染的情况可能比以前怀疑的更为常见。
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