Šašala Martin, Majorová Emeše, Vrzgula Andrej, Fandáková Iveta
Surgery Department, Faculty of Medicine, P.J. Šafárik University, Košice, Slovakia.
Hospital Košice-Šaca, Ltd., 1st Private Hospital, Košice-Šaca, Slovakia.
Ann Coloproctol. 2020 Feb;36(1):12-16. doi: 10.3393/ac.2018.10.15.2. Epub 2020 Mar 3.
The aim of this study was to determine whether a connection exists between Crohn disease and fungi, specifically Candida albicans, because one possible cause of disease is thought to be the presence of fungi in the intra-abdominal cavity. The diagnosis of invasive candidiasis is difficult due to the lack of specific clinical manifestations of the disease. A retrospective evaluation of the presence of invasive candidiasis was done in a group of 54 patients with Crohn disease and in a group of 31 patients who received surgery primarily for right-sided cancer of the colon.
Culture samples were obtained from the wall of the extraluminal portion of the terminal ileum and the adjacent mesenterium, and then sent to the microbiology laboratory for further investigation. Sabouraud agar (SGC2) and chromID Candida agar (CAN2) were used for both short-term (48 hours) and long-term (10 days) cultivation.
Pearson chi-square test revealed a statistically significant difference in the prevalence of fungi and yeast between the 2 groups of patients (χ2 = 4.3873, P < 0.05).
Patients with Crohn disease had a significantly higher prevalence of fungi and yeasts in the intra-abdominal cavity compared with cancer patients.
本研究旨在确定克罗恩病与真菌（特别是白色念珠菌）之间是否存在关联，因为该疾病的一个可能病因被认为是腹腔内存在真菌。由于侵袭性念珠菌病缺乏特异性临床表现，其诊断较为困难。对54例克罗恩病患者和31例主要因右侧结肠癌接受手术的患者进行了侵袭性念珠菌病存在情况的回顾性评估。
从回肠末端肠外部分的肠壁及相邻肠系膜获取培养样本，然后送至微生物实验室进行进一步检测。沙氏琼脂（SGC2）和显色念珠菌琼脂（CAN2）用于短期（48小时）和长期（10天）培养。
Pearson卡方检验显示两组患者之间真菌和酵母菌的患病率存在统计学显著差异（χ2 = 4.3873，P < 0.05）。
与癌症患者相比，克罗恩病患者腹腔内真菌和酵母菌的患病率显著更高。
