State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
FEMS Microbiol Ecol. 2020 Apr 1;96(4). doi: 10.1093/femsec/fiaa038.
Interactions between plants and microbes are involved in biodiversity maintenance, community stability and ecosystem functioning. However, differences in the community and network structures between phyllosphere epiphytic and endophytic bacteria have rarely been investigated. Here, we examined phyllosphere epiphytic and endophytic bacterial communities of six mangrove species using Illumina MiSeq sequencing of the 16S rRNA gene. The results revealed that the community structure of epiphytic and endophytic bacteria was different. Plant identity significantly affected the diversity and community structure of both epiphytic and endophytic bacteria, with a greater effect on the community structure of the former than the latter. Network analysis showed that both plant-epiphytic and plant-endophytic bacterial network structures were characterized by significantly highly specialized and modular but lowly connected and anti-nested properties. Furthermore, the epiphytic bacterial network was more highly specialized and modular but less connected and more strongly anti-nested than the endophytic bacterial network. This study reveals that the phyllosphere epiphytic and endophytic bacterial community structures differ and plant identity has a greater effect on the epiphytic than on the endophytic bacteria, which may provide a comprehensive insight into the role of plant identity in driving the phyllosphere epiphytic and endophytic microbial community structures in mangrove ecosystems.
植物与微生物之间的相互作用参与了生物多样性维持、群落稳定性和生态系统功能。然而，叶际附生和内生细菌群落和网络结构之间的差异很少被研究。在这里，我们使用 Illumina MiSeq 测序 16S rRNA 基因研究了六种红树林物种的叶际附生和内生细菌群落。结果表明，附生和内生细菌的群落结构不同。植物身份显著影响了附生和内生细菌的多样性和群落结构，对前者的影响大于后者。网络分析表明，植物-附生和植物-内生细菌网络结构的特征是高度专业化和模块化，但连接度低且反嵌套。此外，与内生细菌网络相比，附生细菌网络的专业化和模块化程度更高，连接度更低，反嵌套性更强。本研究揭示了叶际附生和内生细菌群落结构的差异，以及植物身份对附生细菌的影响大于内生细菌，这可能为深入了解植物身份在驱动红树林生态系统叶际附生和内生微生物群落结构中的作用提供了全面的认识。
