Biology Department, Syracuse University, Syracuse, New York, USA.
Department of Cell and Developmental Biology, SUNY Upstate Medical School, Syracuse, New York, USA.
Nat Commun. 2020 Mar 9;11(1):1269. doi: 10.1038/s41467-020-15002-8.
Multicellular rosettes are transient epithelial structures that serve as intermediates during diverse organ formation. We have identified a unique contributor to rosette formation in zebrafish Kupffer's vesicle (KV) that requires cell division, specifically the final stage of mitosis termed abscission. KV utilizes a rosette as a prerequisite before forming a lumen surrounded by ciliated epithelial cells. Our studies identify that KV-destined cells remain interconnected by cytokinetic bridges that position at the rosette's center. These bridges act as a landmark for directed Rab11 vesicle motility to deliver an essential cargo for lumen formation, CFTR (cystic fibrosis transmembrane conductance regulator). Here we report that premature bridge cleavage through laser ablation or inhibiting abscission using optogenetic clustering of Rab11 result in disrupted lumen formation. We present a model in which KV mitotic cells strategically place their cytokinetic bridges at the rosette center, where Rab11-associated vesicles transport CFTR to aid in lumen establishment.
多细胞玫瑰花结是一种短暂的上皮结构，在多种器官形成过程中作为中间产物。我们已经鉴定出斑马鱼 Kupffer 囊泡 (KV) 中玫瑰花结形成的一个独特贡献者，它需要细胞分裂，特别是称为胞质分离的有丝分裂的最后阶段。KV 在形成被纤毛上皮细胞包围的腔之前，利用玫瑰花结作为先决条件。我们的研究表明，KV 定向细胞通过位于玫瑰花结中心的胞质分裂桥保持相互连接。这些桥作为一个地标，用于指导 Rab11 囊泡的运动，以输送形成腔所必需的货物，即 CFTR（囊性纤维化跨膜电导调节因子）。在这里，我们报告说，通过激光消融过早地切割桥，或用光遗传学聚集 Rab11 抑制胞质分离，会导致腔形成中断。我们提出了一个模型，其中 KV 有丝分裂细胞在玫瑰花结中心战略性地放置它们的胞质分裂桥，在这里 Rab11 相关囊泡运输 CFTR 以帮助建立腔。
