Xue Yong, Redeuil Karine Meisser, Giménez Esther Campos, Vinyes-Pares Gerard, Zhao Ai, He Tingchao, Yang Xiaoguang, Zheng Yingdong, Zhang Yumei, Wang Peiyu, Thakkar Sagar K
1Department of Nutrition & Food Hygiene, School of Public Health, Peking University Health Science Center, 38 Xueyuan Road, Haidian District, Beijing, 100191 People's Republic of China.
2CAS key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Science, Chaoyang District, Beijing, 100101 People's Republic of China.
BMC Nutr. 2017 Mar 7;3:22. doi: 10.1186/s40795-017-0139-1. eCollection 2017.
Adequate B-vitamins concentrations in human milk are considered to be a prerequisite for healthy development of infants in early life. This study aims to determine the concentrations of B-vitamins in human milk from Chinese women and the relationships between their concentrations and different geographical origin, lactation stages, socioeconomic characteristics, and dietary intake.
Human milk was obtained from 443 healthy lactating women from Beijing ( = 150), Suzhou ( = 146), and Guangzhou ( = 147) cities. Thiamine, riboflavin, vitamin B (nicotinamide and nicotinic acid), and vitamin B (pyridoxal, pyridoxine, and pyridoxamine) in human milk were analyzed by high performance liquid chromatography-tandem mass spectrometry. Pantothenic acid, biotin, and folates in human milk were analyzed by microbiological assay. The information from one 24-h dietary recall and socioeconomic characteristics were collected by interview and structured questionnaire, respectively.
B-vitamins concentrations in human milk varied greatly among individuals. The median concentrations of B-vitamins of 5-11 d, 12-30 d, 31-60 d, 61-120 d, and 121-240 d were respectively as follows: thiamine 3.13, 5.07, 4.28, 5.65, 6.28 (μg/100 g); riboflavin 20.8, 20.2, 11.9, 13.6, 15.6 (μg/100 g); vitamin B 194.0, 300.0, 261.0, 212.5, 218.0 (μg/100 g); pantothenic acid 236.5, 291.0, 254.0, 179.0, 189.0 (μg/100 g); vitamin B 6.34, 7.58, 8.60, 9.34, 10.20 (μg/100 g); biotin 0.462, 0.834, 0.606, 0.523, 0.464 (μg/100 g); folates 0.730, 2.390, 2.440, 2.420, 2.330 (μg/100 g). The levels of B-vitamins presented regional differences and varied significantly among different lactation stages. The inversely associations of thiamine, vitamin B, and folates with maternal BMI were found in multivariate analyses ( < 0.05), as well as higher pantothenic acid, folates, and biotin concentrations in lactating women with supplement intake when compared with those without ( < 0.05). Riboflavin concentrations associated with regular exercise was found in multivariate analyses ( < 0.05).
The present study indicated regional and socioeconomic factors, lactation stage, and supplement intake may influence B-vitamins concentrations of human milk in healthy Chinese mothers. Further studies on accurate and complete analysis of all vitamin forms are crucial for giving a more comprehensive understanding of vitamin status in human milk.
ClinicalTrials.gov, NCT01971671. Registered 13 October 2013.
母乳中充足的B族维生素浓度被认为是婴儿早期健康发育的先决条件。本研究旨在测定中国女性母乳中B族维生素的浓度，以及这些浓度与不同地理来源、哺乳阶段、社会经济特征和饮食摄入之间的关系。
从北京（n = 150）、苏州（n = 146）和广州（n = 147）三个城市的443名健康哺乳期妇女中采集母乳。采用高效液相色谱-串联质谱法分析母乳中的硫胺素、核黄素、维生素B（烟酰胺和烟酸）和维生素B（吡哆醛、吡哆醇和吡哆胺）。采用微生物分析法分析母乳中的泛酸、生物素和叶酸。分别通过访谈和结构化问卷收集24小时饮食回忆信息和社会经济特征。
母乳中B族维生素浓度个体差异很大。5 - 11天、12 - 30天、31 - 60天、61 - 120天和121 - 240天母乳中B族维生素的中位数浓度分别如下：硫胺素3.13、5.07、4.28、5.65、6.28（μg/100g）；核黄素20.8、20.2、11.9、13.6、15.6（μg/100g）；维生素B 194.0、300.0、261.0、212.5、218.0（μg/100g）；泛酸236.5、291.0、254.0、179.0、189.0（μg/100g）；维生素B 6.34、7.58、8.60、9.34、10.20（μg/100g）；生物素0.462、0.834、0.606、0.523、0.
