中国中部刺猬中 的检测。
Detection of in Hedgehogs from Central China.
机构信息
State Key Laboratory of Virology, School of Health Sciences, Wuhan University, Wuhan, China.
Lab Animal Research Center, Hubei University of Chinese Medicine, Wuhan, China.
出版信息
Vector Borne Zoonotic Dis. 2020 Jun;20(6):427-431. doi: 10.1089/vbz.2019.2544. Epub 2020 Mar 5.
is the causative agent of leptospirosis, a zoonotic disease of global importance. To have a better understanding on the host species of , we investigated the prevalence of species in hedgehogs in Central China. Hedgehogs were captured in Hubei Province, China in May and October, 2018. Total DNA was extracted from the kidney tissues of hedgehogs for determining the species by PCR amplification of the , , and genes with genus-specific primers. PCR amplification indicated that the positive rate of hedgehogs to the , , and genes were 19.5% (8/41), 12.2% (5/41), and 9.8% (4/41), respectively. The homology of the partial sequence of , , and genes were 99.0-100% among the strains from hedgehogs. Phylogenetic analysis revealed that species detected in this study clustered together with We detected from hedgehogs in Central China, suggesting hedgehogs are the hosts of
是钩端螺旋体病的病原体，一种具有全球重要性的人畜共患疾病。为了更好地了解的宿主物种，我们调查了中国中部刺猬中 物种的流行情况。2018 年 5 月和 10 月，在中国湖北省捕获了刺猬。从刺猬的肾脏组织中提取总 DNA，并用属特异性引物对 、 和 基因进行 PCR 扩增，以确定 物种。PCR 扩增表明，刺猬对 、 和 基因的阳性率分别为 19.5%（8/41）、12.2%（5/41）和 9.8%（4/41）。从刺猬中分离出的 、 和 基因的部分序列同源性为 99.0-100%。系统发育分析显示，本研究中检测到的 物种与 聚在一起。我们从中国中部的刺猬中检测到了 ，提示刺猬是 的宿主。
Zotero 插件