• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

长链非编码RNA CEBPA-AS1过表达通过Notch信号通路抑制骨肉瘤细胞增殖和迁移并促进其凋亡

lncRNA CEBPA-AS1 Overexpression Inhibits Proliferation and Migration and Stimulates Apoptosis of OS Cells via Notch Signaling.

作者信息

Xia Peng, Gu Rui, Zhang Wei, Sun Yi-Fu

机构信息

Department of Orthopaedics, Second Hospital of Jilin University, Changchun 130041, P.R. China.

Department of Orthopaedics, China-Japan Union Hospital of Jilin University, Changchun 130033, P.R. China.

出版信息

Mol Ther Nucleic Acids. 2020 Mar 6;19:1470-1481. doi: 10.1016/j.omtn.2019.10.017. Epub 2019 Oct 24.

DOI:10.1016/j.omtn.2019.10.017
PMID:32160715
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7056609/
Abstract

Osteosarcoma (OS) is the most common primary bone malignancy derived from primitive bone-forming mesenchymal cells. Long noncoding RNA (lncRNA) expression profiles have been intensively studied for their involvement in OS. Herein, we clarify whether lncRNA CEBPA-AS1 is a regulator of NCOR2 in OS cells. Microarray-based expression analysis identified OS-related differentially expressed lncRNA and predicted microRNAs (miRs) binding to lncRNA and mRNA. lncRNA CEBPA-AS1 and NCOR2 were found to be weakly expressed in OS tissues and cells. Next, functional investigation revealed that lncRNAs CEBPA-AS1 bound to miR-10b-5p to upregulate NCOR2. Following that, gene-targeted knockdown and overexpressed recombinant vectors of lncRNA CEBPA-AS1 and NCOR2 were constructed to explore the effects of lncRNA CEBPA-AS1 and NCOR2 on cell proliferation, differentiation, migration, and apoptosis. Finally, tumor formation was measured in nude mice. lncRNA CEBPA-AS1 overexpression or NCOR2 elevation inhibited cell proliferation and migration, and alkaline phosphatase (ALP) and bone gla protein (BGP) activity, while enhancing apoptosis and tumor formation. Furthermore, NCOR2 was elevated in response to lncRNA CEBPA-AS1 overexpression, thus repressing the Notch signaling pathway. Taken together, lncRNA CEBPA-AS1 overexpression inhibits OS progression through diminishing activation of the Notch signaling pathway via upregulating NCOR2. Therefore, lncRNA CEBPA-AS1 may serve as a molecular target for treating OS.

摘要

骨肉瘤（OS）是源自原始骨形成间充质细胞的最常见原发性骨恶性肿瘤。长链非编码RNA（lncRNA）表达谱因其与骨肉瘤的关系而受到深入研究。在此，我们阐明lncRNA CEBPA-AS1是否为骨肉瘤细胞中NCOR2的调节因子。基于微阵列的表达分析确定了与骨肉瘤相关的差异表达lncRNA，并预测了与lncRNA和mRNA结合的微小RNA（miR）。发现lncRNA CEBPA-AS1和NCOR2在骨肉瘤组织和细胞中表达较弱。接下来，功能研究表明lncRNA CEBPA-AS1与miR-10b-5p结合以上调NCOR2。随后，构建了lncRNA CEBPA-AS1和NCOR2的基因靶向敲低和过表达重组载体，以探讨lncRNA CEBPA-AS1和NCOR2对细胞增殖、分化、迁移和凋亡的影响。最后，在裸鼠中测量肿瘤形成。lncRNA CEBPA-AS1过表达或NCOR2升高抑制细胞增殖和迁移，以及碱性磷酸酶（ALP）和骨钙蛋白（BGP）活性，同时增强凋亡和肿瘤形成。此外，NCOR2因lncRNA CEBPA-AS1过表达而升高，从而抑制Notch信号通路。综上所述，lncRNA CEBPA-AS1过表达通过上调NCOR2减少Notch信号通路的激活来抑制骨肉瘤进展。因此，lncRNA CEBPA-AS1可能作为治疗骨肉瘤的分子靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fed0/7056609/cc19a93df98a/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fed0/7056609/d119ee68c9f8/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fed0/7056609/b11243c0eb9f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fed0/7056609/187f716224c3/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fed0/7056609/dfa721a7f2fb/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fed0/7056609/e7688e4d8256/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fed0/7056609/fd47ff48a38d/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fed0/7056609/63b1a98b3ac2/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fed0/7056609/cc19a93df98a/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fed0/7056609/d119ee68c9f8/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fed0/7056609/b11243c0eb9f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fed0/7056609/187f716224c3/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fed0/7056609/dfa721a7f2fb/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fed0/7056609/e7688e4d8256/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fed0/7056609/fd47ff48a38d/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fed0/7056609/63b1a98b3ac2/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fed0/7056609/cc19a93df98a/gr7.jpg

相似文献

1
lncRNA CEBPA-AS1 Overexpression Inhibits Proliferation and Migration and Stimulates Apoptosis of OS Cells via Notch Signaling.长链非编码RNA CEBPA-AS1过表达通过Notch信号通路抑制骨肉瘤细胞增殖和迁移并促进其凋亡
Mol Ther Nucleic Acids. 2020 Mar 6;19:1470-1481. doi: 10.1016/j.omtn.2019.10.017. Epub 2019 Oct 24.
2
Silencing of long noncoding RNA SBF2-AS1 inhibits proliferation, migration and invasion and contributes to apoptosis in osteosarcoma cells by upregulating microRNA-30a to suppress FOXA1 expression.长链非编码 RNA SBF2-AS1 的沉默通过上调 microRNA-30a 抑制 FOXA1 表达，抑制骨肉瘤细胞的增殖、迁移和侵袭并促进细胞凋亡。
Cell Cycle. 2019 Oct;18(20):2727-2741. doi: 10.1080/15384101.2019.1656478. Epub 2019 Aug 21.
3
Downregulated Long Non-Coding RNA MSC-AS1 Inhibits Osteosarcoma Progression and Increases Sensitivity to Cisplatin by Binding to MicroRNA-142.下调的长链非编码 RNA MSC-AS1 通过与 microRNA-142 结合抑制骨肉瘤进展并增加顺铂敏感性
Med Sci Monit. 2020 Mar 10;26:e921594. doi: 10.12659/MSM.921594.
4
LncRNA FOXD2-AS1 knockdown inhibits the resistance of human osteosarcoma cells to cisplatin by inhibiting miR-143 expression.长链非编码 RNA FOXD2-AS1 敲低通过抑制 miR-143 的表达抑制人骨肉瘤细胞对顺铂的耐药性。
Eur Rev Med Pharmacol Sci. 2021 Jan;25(2):678-686. doi: 10.26355/eurrev_202101_24629.
5
LncRNA CEBPA-AS1 alleviates cerebral ischemia-reperfusion injury by sponging miR-340-5p regulating APPL1/LKB1/AMPK pathway.长链非编码 RNA CEBPA-AS1 通过海绵吸附 miR-340-5p 调节 APPL1/LKB1/AMPK 通路减轻脑缺血再灌注损伤。
FASEB J. 2022 Jan;36(1):e22075. doi: 10.1096/fj.202100826RR.
6
Long non-coding RNA NR2F2-AS1 regulates human osteosarcoma growth and metastasis through miR-425-5p-mediated HMGB2.长非编码 RNA NR2F2-AS1 通过 miR-425-5p 介导的 HMGB2 调控人骨肉瘤的生长和转移。
Int J Clin Oncol. 2022 Dec;27(12):1891-1903. doi: 10.1007/s10147-022-02245-2. Epub 2022 Oct 21.
7
Knockdown of lncRNA C5orf66-AS1 inhibits osteosarcoma cell proliferation and invasion via miR-149-5p upregulation.lncRNA C5orf66-AS1的敲低通过上调miR-149-5p抑制骨肉瘤细胞的增殖和侵袭。
Oncol Lett. 2021 Nov;22(5):757. doi: 10.3892/ol.2021.13018. Epub 2021 Sep 2.
8
LncRNA AFAP1-AS1 promotes tumorigenesis and epithelial-mesenchymal transition of osteosarcoma through RhoC/ROCK1/p38MAPK/Twist1 signaling pathway.长链非编码 RNA AFAP1-AS1 通过 RhoC/ROCK1/p38MAPK/Twist1 信号通路促进骨肉瘤的发生发展和上皮间质转化。
J Exp Clin Cancer Res. 2019 Aug 23;38(1):375. doi: 10.1186/s13046-019-1363-0.
9
LncRNA SOX21-AS1 Promotes the Growth and Invasiveness of Osteosarcoma Cells Through miR-7-5p/IRS2 Regulatory Network.长链非编码 RNA SOX21-AS1 通过 miR-7-5p/IRS2 调控网络促进骨肉瘤细胞的生长和侵袭。
Arch Med Res. 2021 Apr;52(3):294-303. doi: 10.1016/j.arcmed.2020.11.007. Epub 2020 Dec 17.
10
Actin filament-associated protein 1-antisense RNA1 promotes the development and invasion of tongue squamous cell carcinoma via the AFAP1-AS1/miR-133a-5p/ZIC2 axis.肌动蛋白丝相关蛋白 1 反义 RNA1 通过 AFAP1-AS1/miR-133a-5p/ZIC2 轴促进舌鳞癌细胞的发展和侵袭。
J Gene Med. 2024 Jan;26(1):e3654. doi: 10.1002/jgm.3654.

引用本文的文献

1
Exosomal ncRNAs in liquid biopsy: a new paradigm for early cancer diagnosis and monitoring.液体活检中的外泌体非编码RNA：早期癌症诊断与监测的新范式
Front Oncol. 2025 Jul 9;15:1615433. doi: 10.3389/fonc.2025.1615433. eCollection 2025.
2
Emerging roles of long non-coding RNAs in osteosarcoma.长链非编码RNA在骨肉瘤中的新兴作用
Front Mol Biosci. 2024 Mar 7;11:1327459. doi: 10.3389/fmolb.2024.1327459. eCollection 2024.
3
MFNG is an independent prognostic marker for osteosarcoma.MFNG 是骨肉瘤的一个独立预后标志物。

本文引用的文献

1
Loss of function of NCOR1 and NCOR2 impairs memory through a novel GABAergic hypothalamus-CA3 projection.NCOR1 和 NCOR2 功能丧失通过新型 GABA 能下丘脑-CA3 投射损害记忆。
Nat Neurosci. 2019 Feb;22(2):205-217. doi: 10.1038/s41593-018-0311-1. Epub 2019 Jan 21.
2
Retraction: Downregulation of Notch-1 Contributes to Cell Growth Inhibition and Apoptosis in Pancreatic Cancer Cells.撤回声明：Notch-1的下调有助于抑制胰腺癌细胞的生长和诱导其凋亡。
Mol Cancer Ther. 2018 Oct;17(10):2268. doi: 10.1158/1535-7163.MCT-18-0524.
3
LncRNA-p21 inhibited the proliferation of osteosarcoma cells via the miR-130b/PTEN/AKT signaling pathway.
Eur J Med Res. 2023 Jul 26;28(1):256. doi: 10.1186/s40001-023-01139-x.
4
PCB: A pseudotemporal causality-based Bayesian approach to identify EMT-associated regulatory relationships of AS events and RBPs during breast cancer progression.PCB：一种基于伪时间因果关系的贝叶斯方法，用于识别乳腺癌进展过程中 AS 事件和 RBPs 的 EMT 相关调控关系。
PLoS Comput Biol. 2023 Mar 17;19(3):e1010939. doi: 10.1371/journal.pcbi.1010939. eCollection 2023 Mar.
5
Notch-associated lncRNAs profiling circuiting epigenetic modification in colorectal cancer.Notch相关长链非编码RNA对结直肠癌表观遗传修饰的调控机制
Cancer Cell Int. 2022 Oct 13;22(1):316. doi: 10.1186/s12935-022-02736-2.
6
Navigating the genomic instability mine field of osteosarcoma to better understand implications of non-coding RNAs.探索骨肉瘤的基因组不稳定性雷区，以更好地理解非编码RNA的影响。
Biocell. 2022 Jun 13;46(10):2177-2193. doi: 10.32604/biocell.2022.020141.
7
LncRNA CEBPA-AS1 knockdown prevents neuronal apoptosis against oxygen glucose deprivation/reoxygenation by regulating the miR-455/GPER1 axis.长链非编码 RNA CEBPA-AS1 敲低通过调节 miR-455/GPER1 轴防止神经元在氧葡萄糖剥夺/复氧后凋亡。
Metab Brain Dis. 2022 Mar;37(3):677-688. doi: 10.1007/s11011-021-00881-7. Epub 2022 Jan 28.
8
Oncogenic Role of Exosomal Circular and Long Noncoding RNAs in Gastrointestinal Cancers.外泌体环状和长非编码 RNA 在胃肠道癌症中的致癌作用。
Int J Mol Sci. 2022 Jan 15;23(2):930. doi: 10.3390/ijms23020930.
9
Long noncoding RNA Gm44593 attenuates oxidative stress from age-related hearing loss by regulating miR-29b/WNK1.长链非编码 RNA Gm44593 通过调节 miR-29b/WNK1 减轻与年龄相关的听力损失中的氧化应激。
Bioengineered. 2022 Jan;13(1):573-582. doi: 10.1080/21655979.2021.2012062.
10
Long noncoding RNAs: fine-tuners hidden in the cancer signaling network.长链非编码RNA：隐藏在癌症信号网络中的微调器。
Cell Death Discov. 2021 Oct 11;7(1):283. doi: 10.1038/s41420-021-00678-8.
长链非编码 RNA-p21 通过 miR-130b/PTEN/AKT 信号通路抑制骨肉瘤细胞的增殖。
Biomed Pharmacother. 2018 Jan;97:911-918. doi: 10.1016/j.biopha.2017.11.014. Epub 2017 Nov 7.
4
Mir-150 Up-Regulates Glut1 and Increases Glycolysis in Osteosarcoma Cells.Mir-150上调骨肉瘤细胞中的Glut1并增加糖酵解
Asian Pac J Cancer Prev. 2017 Apr 1;18(4):1127-1131. doi: 10.22034/APJCP.2017.18.4.1127.
5
The combination of circulating long noncoding RNAs AK001058, INHBA-AS1, MIR4435-2HG, and CEBPA-AS1 fragments in plasma serve as diagnostic markers for gastric cancer.血浆中循环长链非编码RNA AK001058、INHBA-AS1、MIR4435-2HG和CEBPA-AS1片段的组合可作为胃癌的诊断标志物。
Oncotarget. 2017 Mar 28;8(13):21516-21525. doi: 10.18632/oncotarget.15628.
6
Notch as a tumour suppressor.Notch 作为一种肿瘤抑制因子。
Nat Rev Cancer. 2017 Mar;17(3):145-159. doi: 10.1038/nrc.2016.145. Epub 2017 Feb 3.
7
Long non-coding RNA regulates proliferation and invasion via activating Notch signalling pathway in retinoblastoma.长链非编码RNA通过激活视网膜母细胞瘤中的Notch信号通路来调节细胞增殖和侵袭。
J Biosci. 2016 Dec;41(4):677-687. doi: 10.1007/s12038-016-9636-7.
8
Review of Osteosarcoma and Current Management.骨肉瘤综述与当前治疗方法
Rheumatol Ther. 2016 Dec;3(2):221-243. doi: 10.1007/s40744-016-0046-y. Epub 2016 Oct 19.
9
Molecular genetics of osteosarcoma.骨肉瘤的分子遗传学。
Bone. 2017 Sep;102:69-79. doi: 10.1016/j.bone.2016.10.017. Epub 2016 Oct 17.
10
Nuclear receptor corepressors Ncor1 and Ncor2 (Smrt) are required for retinoic acid-dependent repression of Fgf8 during somitogenesis.在体节发生过程中，视黄酸依赖性抑制Fgf8需要核受体共抑制因子Ncor1和Ncor2（Smrt）。
Dev Biol. 2016 Oct 1;418(1):204-215. doi: 10.1016/j.ydbio.2016.08.005. Epub 2016 Aug 6.