Bukasa K S, Sindic C J, Bodeus M, Burtonboy G, Laterre C, Sonnet J
Department of Neurology, St-Luc Hospital, University of Louvain, Brussels, Belgium.
J Neurol Neurosurg Psychiatry. 1988 Aug;51(8):1063-8. doi: 10.1136/jnnp.51.8.1063.
CSF and serum samples from 16 AIDS patients were tested for the presence of anti-HIV antibodies either by classical serological methods or by an immunoblot technique based on agarose gel isoelectric focusing and transfer of the specific IgG antibodies onto HIV antigens-loaded nitrocellulose sheets. This method enabled the demonstration of an intrathecal synthesis of anti-HIV oligoclonal IgG antibodies, often superimposed on diffuse polyclonal production, in 14 patients. The two negative cases were devoid of neurological signs or symptoms. However, two patients classified in stage II of the disease (asymptomatic infection) displayed an intrathecal synthesis of anti-HIV antibodies.
采用经典血清学方法或基于琼脂糖凝胶等电聚焦及将特异性IgG抗体转移至负载HIV抗原的硝酸纤维素膜上的免疫印迹技术，检测了16例艾滋病患者脑脊液（CSF）和血清样本中抗HIV抗体的存在情况。该方法能够证实14例患者存在鞘内抗HIV寡克隆IgG抗体合成，且常叠加于弥漫性多克隆产生之上。2例阴性病例无神经体征或症状。然而，2例处于疾病II期（无症状感染）的患者显示存在鞘内抗HIV抗体合成。
Zotero 插件