Qi Liying, Chen Ling, Wang Chuansen, Zhang Shaoling, Yang Yingjie, Liu Jianlong, Li Dingli, Song Jiankun, Wang Ran
College of Horticulture, Qingdao Key Lab of Genetic Improvement and Breeding of Horticultural Plants, Qingdao Agricultural University, Qingdao 266109, China.
Institute of Soil and Fertilizer & Resource and Environment, Jiangxi Academy of Agriculture Sciences, Nanchang 330200, China.
Plants (Basel). 2020 Mar 10;9(3):349. doi: 10.3390/plants9030349.
PIN-FORMED (PIN) encodes a key auxin polar transport family that plays a crucial role in the outward transport of auxin and several growth and development processes, including dwarfing trees. We identified a dwarfing pear rootstock 'OHF51' (), which limits the growth vigor of the 'Xueqing' ( × ) scion, and isolated 14 putative PbPINs from the pear . The phylogenic relationships, structure, promoter regions, and expression patterns were analyzed. PbPINs were classified into two main groups based on the protein domain structure and categorized into three major groups using the neighbor-joining algorithm. Promoter analysis demonstrated that might be closely related to plant growth and development. Through quantitative real-time PCR (qRT-PCR) analysis, we found that the expression patterns of 14 varied upon exposure to different organs in dwarfing and vigorous stocks, 'OHF51' and 'QN101' (), indicating that they might play varying roles in different tissues and participated in the regulation of growth vigor. These results provide fundamental insights into the characteristics and evolution of the PINs family, as well as the possible relationship between dwarfing ability and auxin polar transport.
PIN-FORMED（PIN）编码一个关键的生长素极性运输家族，该家族在生长素的外向运输以及包括矮化树木在内的多个生长和发育过程中起着至关重要的作用。我们鉴定出一种矮化梨砧木‘OHF51’（），它限制了‘雪青’（×）接穗的生长活力，并从梨中分离出14个假定的PbPINs。对其系统发育关系、结构、启动子区域和表达模式进行了分析。基于蛋白质结构域结构，PbPINs被分为两个主要组，并使用邻接法算法分为三个主要组。启动子分析表明，可能与植物生长发育密切相关。通过定量实时PCR（qRT-PCR）分析，我们发现14个在矮化和生长旺盛的砧木‘OHF51’和‘QN101’（）中，暴露于不同器官时表达模式有所不同，这表明它们可能在不同组织中发挥不同作用，并参与生长活力的调节。这些结果为PINs家族的特征和进化以及矮化能力与生长素极性运输之间的可能关系提供了基本见解。
