Chinese PLA Medical School, Beijing 100853, China; Fifth Medical Center of Chinese PLA General Hospital, Beijing 100039, China.
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Infect Genet Evol. 2020 Aug;82:104279. doi: 10.1016/j.meegid.2020.104279. Epub 2020 Mar 10.
It is important to identify viruses in animals because most infectious diseases in humans are caused by viruses of zoonotic origin. African green monkey is a widely used non-human primate model in biomedical investigations. In this study, total RNAs were extracted from stool samples of 10 African green monkeys with diarrhea. High-throughput sequencing was used to characterize viromes. PCR and Sanger sequencing were used to determine the full genome sequences. Great viral diversity was observed. The dominant viruses were enteroviruses and picobirnaviruses. Six enterovirus genomes and a picobirnavirus RNA-dependent RNA polymerase sequence were characterized. Five enteroviruses belonged to two putative new genotypes of species Enterovirus J. One enterovirus belonged to EV-A92. The picobirnavirus RNA-dependent RNA polymerase sequence had the highest nucleotide similarity (93.48%) with human picobirnavirus isolate GPBV6C2. The present study helped to identify the potential zoonotic viruses in African green monkeys. Further investigations are required to elucidate their pathogenic roles in animals and humans.
鉴定动物病毒很重要，因为人类大多数传染病都是由动物源的病毒引起的。非洲绿猴是生物医学研究中广泛使用的非人类灵长类动物模型。在这项研究中，从 10 只患有腹泻的非洲绿猴的粪便样本中提取了总 RNA。高通量测序用于描述病毒组。PCR 和 Sanger 测序用于确定全基因组序列。观察到了很大的病毒多样性。主要病毒是肠道病毒和微小 RNA 病毒。鉴定了六个肠道病毒基因组和一个微小 RNA 病毒 RNA 依赖性 RNA 聚合酶序列。五种肠道病毒属于两个假定的新型属肠道病毒 J 种。一种肠道病毒属于 EV-A92。微小 RNA 病毒 RNA 依赖性 RNA 聚合酶序列与人类微小 RNA 病毒分离株 GPBV6C2 的核苷酸相似性最高（93.48%）。本研究有助于鉴定非洲绿猴中的潜在人畜共患病病毒。需要进一步研究来阐明它们在动物和人类中的致病作用。
