Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, 06560, Ankara, Turkey.
Mikrochim Acta. 2020 Mar 12;187(4):223. doi: 10.1007/s00604-020-4206-y.
An innovative and portable design to fabricate an electrochemical sensor based on metallic phase MoS (1T-MoS) decorated with shape-dependent gold nanostructures for the determination of doxorubicin (DOX) is presented. In this context, homogenous and uniform single-crystal gold nanospheres (AuNSPs) and nanorods (AuNRDs) were firstly synthesized by seeded growth approach. Afterwards, AuNSPs and AuNRDs were anchored on 1T-MoS surfaces to construct the desired electrochemical sensing platform towards the DOX assay. 1T-MoS was exfoliated by metal intercalation process using NaK metal alloys. The structure and surface morphology of 1T-MoS, AuNSPs, and AuNRDs were characterized by XPS, Raman, UV-vis, TEM, and SEM. The electrochemical behavior of DOX using various MoS-based electrochemical sensors prepared on screen-printed electrode (SPE) was examined by cyclic voltammetry and adsorptive stripping differential pulse voltammetry. The electrocatalytic efficiency of AuNRDs on 1T-MoS was also compared with that of AuNSPs on 1T-MoS, and it showed much better electrocatalytic activity towards the DOX. A nanocomposite prepared with AuNRDs and 1T-MoS on SPE (AuNRDs/1T-MoS/SPE) exhibited a linear relationship between peak current and DOX concentration in the range 0.01-9.5 μM with a detection limit of 2.5 nM. The AuNRDs/1T-MoS/SPE was successfully applied to the sensitive and rapid determination of DOX in spiked human serum samples with satisfactory recoveries in the range 99.2-100.8%. Graphical abstract Schematic representation of a portable design for electrochemical sensor based on shape-controlled gold nanostructures decorated on metallic phase molybdenum disulfide (1T-MoS) towards the sensitive determination of doxorubicin.
呈现了一种基于金属相 MoS（1T-MoS）的电化学传感器的创新性和便携式设计，该传感器通过形状依赖性金纳米结构进行修饰，用于测定阿霉素（DOX）。在这种情况下，首先通过种子生长方法合成了均匀且单分散的单晶金纳米球（AuNSPs）和纳米棒（AuNRDs）。然后，将 AuNSPs 和 AuNRDs 锚定在 1T-MoS 表面上，以构建用于 DOX 测定的理想电化学传感平台。1T-MoS 通过使用 NaK 金属合金的金属插层过程进行剥离。通过 XPS、拉曼、紫外-可见、TEM 和 SEM 对 1T-MoS、AuNSPs 和 AuNRDs 的结构和表面形态进行了表征。通过循环伏安法和吸附剥离差分脉冲伏安法研究了各种基于 MoS 的电化学传感器在丝网印刷电极（SPE）上制备的 DOX 的电化学行为。还比较了 AuNRDs 在 1T-MoS 上的电催化效率与 AuNSPs 在 1T-MoS 上的电催化效率，结果表明 AuNRDs 对 DOX 具有更好的电催化活性。在 SPE 上制备的 AuNRDs/1T-MoS 纳米复合材料（AuNRDs/1T-MoS/SPE）在 0.01-9.5 μM 范围内表现出与 DOX 浓度之间的线性关系，检测限为 2.5 nM。成功地将 AuNRDs/1T-MoS/SPE 应用于含有满意回收率（99.2-100.8%）的加标人血清样品中 DOX 的灵敏快速测定。
