Somov Institute of Epidemiology and Microbiology, Vladivostok 690087, Russia.
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Vladivostok 690022, Russia.
Mar Drugs. 2020 Mar 11;18(3):155. doi: 10.3390/md18030155.
Thе study presents the results of a comparative evaluation of the effect of structural modifications of fucoidans from the brown alga Fucus evanescens (native, highly purified рroduct of fucoidan enzymatic hydrolysis, a new regular 1→3;1→4-α-L-fucan, sulphated mainly at C2 and acetylated at C4 of the fucose residue) on the effector functions of innate and adaptive immunity cells in vitro and in vivo. Using flow cytometry, we found that all examined fucoidans induce the maturation of dendritic cells, enhance the ability of neutrophils to migrate and adhere, activate monocytes and enhance their antigen-presenting functions, and increase the cytotoxic potential of natural killers. Fucoidans increase the production of hepatitis B virus (HBs) specific IgG and cytokine Th1 (IFN-γ, TNF-α) and Th2 (IL-4) profiles in vivo. The data obtained suggest that in vitro and in vivo adjuvant effects of the products of fucoidan enzymatic hydrolysis with regular structural characteristics are comparable to those of the native fucoidan. Based on these data, the products of fucoidan enzymatic hydrolysis can be considered as an effective and safe candidate adjuvant to improve the efficacy of prophylactic and therapeutic vaccines.
该研究展示了对来自褐藻墨角藻（天然、高度纯化的褐藻糖胶酶解产物、新型规则 1→3；1→4-α-L-岩藻聚糖、主要在 C2 位硫酸化和 C4 位乙酰化的岩藻糖残基）结构修饰对固有和适应性免疫细胞体外和体内效应功能的影响的比较评估结果。通过流式细胞术，我们发现所有研究的褐藻糖胶均诱导树突状细胞成熟，增强中性粒细胞的迁移和黏附能力，激活单核细胞并增强其抗原呈递功能，并增加自然杀伤细胞的细胞毒性潜力。褐藻糖胶增加体内乙型肝炎病毒（HBV）特异性 IgG 和细胞因子 Th1（IFN-γ、TNF-α）和 Th2（IL-4）谱的产生。所得数据表明，具有规则结构特征的褐藻糖胶酶解产物的体外和体内佐剂作用可与天然褐藻糖胶相媲美。基于这些数据，褐藻糖胶酶解产物可以被认为是一种有效和安全的候选佐剂，以提高预防性和治疗性疫苗的疗效。
