Li Shaokun, Li Li, Jiang Yang, Wu Jun, Sun Honghua, Zhao Mingzhu, Jiang Yue, Zhu Lei, Wang Yanfang, Su Yingjie, Wang Kangyu, Wang Yi, Zhang Meiping
College of Life Science, Jilin Agricultural University, Changchun, Jilin, 130118, China.
Research Center Ginseng Genetic Resources Development and Utilization, Changchun, Jilin, 130118, China.
Plants (Basel). 2020 Mar 11;9(3):354. doi: 10.3390/plants9030354.
( promoter binding protein-like) gene family is specific transcription factor in the plant that have an important function for plant growth and development. Although the gene family has been widely studied and reported in many various plant species from gymnosperm to angiosperm, there are no systematic studies and reports about the gene family in C. A. Meyer. In this study, we conducted transcriptome-wide identification, evolutionary analysis, structure analysis, and expression characteristics analysis of gene family in by bioinformatics. We annotated the gene family and found that they might involve in multiple functions including encoding structural proteins, but the main function were still focused on the binding function The result showed that 106 transcripts were classified into two clades - A and B, both of which respectively consisted of three groups. Besides, we profiled transcripts' genotypic, temporal, and spatial expression characteristics. Furthermore, we calculated the correlation of transcripts in the 14 tissues of a 4 years old ginseng and 42 farmers' cultivars farmers' cultivars of 4 years old ginsengs' roots with both results showing that transcripts formed a single network, which indicated that s inter-coordinated when performing their functions. What's more, we found that most transcripts tended to express in older ginseng instead of younger ginseng, which was not only reflected in the expression of more types of transcripts in older ginseng, but also in the higher expression of transcripts in older ginseng. Additionally, we found that four transcripts were only massively expressed in roots. According to PgSPL transcripts' expression characteristics, we found that PgSPL23-35 and PgSPL24-09 were most proper two transcripts to further study as ginseng age's molecular marker. These results provide the basis for further elucidation of the transcripts' biological function in ginseng and ginseng genetics improvement and gene breeding in the future.
（类启动子结合蛋白）基因家族是植物中的特异性转录因子，对植物生长发育具有重要作用。尽管该基因家族已在从裸子植物到被子植物的许多不同植物物种中得到广泛研究和报道，但关于C. A. Meyer中该基因家族尚无系统的研究和报道。在本研究中，我们通过生物信息学对C. A. Meyer中该基因家族进行了全转录组鉴定、进化分析、结构分析和表达特征分析。我们注释了该基因家族，发现它们可能参与多种功能，包括编码结构蛋白，但其主要功能仍集中在结合功能上。结果表明，106个转录本被分为两个进化枝——A和B，两者分别由三组组成。此外，我们分析了转录本的基因型、时间和空间表达特征。此外，我们计算了4年生人参14个组织和42个农家品种4年生人参根中转录本的相关性，结果均表明转录本形成了一个单一网络，这表明它们在执行功能时相互协调。更重要的是，我们发现大多数转录本倾向于在较老的人参中表达而非较年轻的人参，这不仅体现在较老人参中更多类型转录本的表达上，还体现在较老人参中转录本的更高表达上。此外，我们发现四个转录本仅在根中大量表达。根据PgSPL转录本的表达特征，我们发现PgSPL23 - 35和PgSPL24 - 09是作为人参年龄分子标记进一步研究的最合适的两个转录本。这些结果为进一步阐明人参中转录本的生物学功能以及未来人参的遗传改良和基因育种提供了依据。
