Ou Yun-Jing, Ren Qiao-Qiao, Fang Shu-Ting, Wu Ji-Guo, Jiang Yun-Xia, Chen Yi-Ran, Zhong Yi, Wang De-Dong, Zhang Guo-Xia
Department of Environmental Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China.
Department of Water Hygiene, Guangzhou Center for Disease Control and Prevention, Guangzhou, China.
Front Genet. 2020 Feb 26;11:119. doi: 10.3389/fgene.2020.00119. eCollection 2020.
CF11 was originally isolated from the gut of healthy humans. To determine the underlying molecular and genetic mechanisms of the probiotic potential of CF11, we performed complete genome sequencing, annotation, and comparative genome analysis. The complete genome of . CF11 comprised of 1,997,720 bp, with a DNA G+C content of 38.21 mol% containing 1982 protein coding genes and 16 rRNA operons. We found that 1206 genes (56.05%) were assigned a putative function using the gene ontology (GO) resource. The gene products of CF11 were primarily concentrated in molecular function and biological processes, such as catalysis, binding, metabolism, and cellular processes. Furthermore, 1,365 (68.87%) genes were assigned an illative function using COGs. CF11 proteins were associated with carbohydrate transport and metabolism, and amino acid transport and metabolism. This indicates that CF11 bacteria can perform active energy exchange. We classified 1,111 (56.05%) genes into six KEGG functional categories; fructose-bisphosphate aldolase and the phosphoenol pyruvate:phosphotransferase system (PTS), which are necessary in producing short-chain fatty acids (SCFAs), were excited in the carbohydrate metabolic pathway. This suggests that . CF11 produces SCFAs glycolysis. The genomic island revealed that some regions contain fragments of antibiotic resistance and bacteriostatic genes. In addition, ANI analysis showed that . CF11 had the closest relationship with . 159469, with an average nucleotide consistency of 98.03%. Taken together, the present study offers further insights into the functional and potential role of . CF11 in health care.
CF11最初是从健康人的肠道中分离出来的。为了确定CF11益生菌潜力的潜在分子和遗传机制，我们进行了全基因组测序、注释和比较基因组分析。CF11的全基因组由1,997,720个碱基对组成，DNA的G+C含量为38.21摩尔%，包含1982个蛋白质编码基因和16个rRNA操纵子。我们发现，使用基因本体论（GO）资源，1206个基因（56.05%）被赋予了推定功能。CF11的基因产物主要集中在分子功能和生物学过程中，如催化、结合、代谢和细胞过程。此外，使用COGs为1365个（68.87%）基因赋予了推断功能。CF11蛋白与碳水化合物运输和代谢以及氨基酸运输和代谢相关。这表明CF11细菌可以进行活跃的能量交换。我们将1111个（56.05%）基因分类为六个KEGG功能类别；在碳水化合物代谢途径中，产生短链脂肪酸（SCFAs）所必需的果糖-1,6-二磷酸醛缩酶和磷酸烯醇丙酮酸：磷酸转移酶系统（PTS）被激活。这表明CF11通过糖酵解产生SCFAs。基因组岛显示，一些区域包含抗生素抗性和抑菌基因的片段。此外，ANI分析表明，CF11与159469的关系最为密切，平均核苷酸一致性为98.03%。综上所述，本研究为CF11在医疗保健中的功能和潜在作用提供了进一步的见解。
