Klimiuk Anna, Zalewska Anna, Sawicki Robert, Knapp Małgorzata, Maciejczyk Mateusz
Experimental Dentistry Laboratory, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, Poland.
Department of Cardiology, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, Poland.
J Clin Med. 2020 Mar 12;9(3):769. doi: 10.3390/jcm9030769.
The aim of the study was to evaluate the rate of reactive oxygen species (ROS) production, antioxidant barrier, and oxidative damage in non-stimulated (NWS) and stimulated (SWS) saliva as well as plasma/erythrocytes of 50 patients with chronic heart failure (HF) divided into the two subgroups: NYHA II (33 patients) and NYHA III (17 patients). The activity of superoxide dismutase and catalase was statistically increased in NWS of HF patients as compared to healthy controls. The free radical formation, total oxidant status, level of uric acid, advanced glycation end products (AGE), advanced oxidation protein products and malondialdehyde was significantly elevated in NWS, SWS, and plasma of NYHA III patients as compared to NYHA II and controls. We were the first to demonstrate that with the progression of HF, disturbances of enzymatic and non-enzymatic antioxidant defense, and oxidative damage to proteins and lipids occur at both central (plasma/erythrocytes) and local (saliva) levels. In the study group, we also observed a decrease in saliva secretion, total salivary protein and salivary amylase activity compared to age- and gender-matched control group, which indicates secretory dysfunction of salivary glands in patients with HF. Salivary AGE may be a potential biomarker in differential diagnosis of HF.
本研究的目的是评估50例慢性心力衰竭（HF）患者分为两个亚组：纽约心脏协会（NYHA）II级（33例）和NYHA III级（17例），其未刺激唾液（NWS）和刺激唾液（SWS）以及血浆/红细胞中的活性氧（ROS）产生率、抗氧化屏障和氧化损伤情况。与健康对照组相比，HF患者NWS中超氧化物歧化酶和过氧化氢酶的活性在统计学上有所增加。与NYHA II级患者和对照组相比，NYHA III级患者的NWS、SWS和血浆中的自由基形成、总氧化剂状态、尿酸水平、晚期糖基化终产物（AGE）、晚期氧化蛋白产物和丙二醛显著升高。我们首次证明，随着HF的进展，酶促和非酶促抗氧化防御的紊乱以及蛋白质和脂质的氧化损伤在中枢（血浆/红细胞）和局部（唾液）水平均会发生。在研究组中，与年龄和性别匹配的对照组相比，我们还观察到唾液分泌、总唾液蛋白和唾液淀粉酶活性降低，这表明HF患者唾液腺存在分泌功能障碍。唾液AGE可能是HF鉴别诊断的潜在生物标志物。
