Sawczuk Beata, Maciejczyk Mateusz, Sawczuk-Siemieniuk Magdalena, Posmyk Renata, Zalewska Anna, Car Halina
Department of Prosthodontics, Medical University of Bialystok, Sklodowskiej 24a, 15-276 Bialystok, Poland.
Department of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, Mickiewicza 2C, 15-222 Bialystok, Poland.
Cancers (Basel). 2019 Oct 8;11(10):1501. doi: 10.3390/cancers11101501.
Oxidative stress plays a key role in breast cancer progression. However, little is still known about the relationship between the mutation, the incidence of breast cancer and oral homeostasis. This is the first study to evaluate the secretory function of salivary glands, biomarkers of redox balance, and oxidative damage to proteins and lipids in the saliva of subjects with the mutation. Ninety eight women were enrolled in the study and allocated to four groups based on molecular DNA testing: generally healthy patients without the mutation, patients with breast cancer but without the mutation, generally healthy patients with the mutation, and patients with both breast cancer and the mutation. We demonstrated that saliva from breast cancer patients with the mutation is characterized by enhanced antioxidant capacity and a higher degree of oxidative damage to proteins and lipids. The mutation can cause a predisposition to early salivary gland dysfunction, both in patients with breast cancer and in healthy individuals, leading to a decrease in salivary proteins. Using cluster analysis, we showed that salivary peroxidase, advanced glycation end-products (AGE), total antioxidant status (TAS) and malondialdehyde (MDA) may have particular clinical significance in non-invasive diagnostics of breast cancer.
氧化应激在乳腺癌进展中起关键作用。然而，关于该突变、乳腺癌发病率与口腔内稳态之间的关系仍知之甚少。这是第一项评估携带该突变受试者唾液腺分泌功能、氧化还原平衡生物标志物以及唾液中蛋白质和脂质氧化损伤的研究。98名女性参与了该研究，并根据分子DNA检测分为四组：无该突变的一般健康患者、患有乳腺癌但无该突变的患者、有该突变的一般健康患者以及同时患有乳腺癌和该突变的患者。我们证明，携带该突变的乳腺癌患者的唾液具有抗氧化能力增强以及蛋白质和脂质氧化损伤程度更高的特征。该突变可导致乳腺癌患者和健康个体均易出现早期唾液腺功能障碍，从而导致唾液蛋白减少。通过聚类分析，我们表明唾液过氧化物酶、晚期糖基化终产物（AGE）、总抗氧化状态（TAS）和丙二醛（MDA）在乳腺癌的非侵入性诊断中可能具有特殊的临床意义。
