Morawska Katarzyna, Maciejczyk Mateusz, Popławski Łukasz, Popławska-Kita Anna, Kretowski Adam, Zalewska Anna
Department of Restorative Dentistry, Medical University of Bialystok, 24A M. Sklodowskiej-Curie Street, 15-276 Bialystok, Poland.
Department of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, 2c Mickiewicza Street, 15-022 Bialystok, Poland.
J Clin Med. 2020 Jul 3;9(7):2102. doi: 10.3390/jcm9072102.
Hashimoto's thyroiditis (HT) is one of the most common autoimmune diseases. Although HT is inextricably linked to oxidative stress, there have been no studies assessing salivary redox homeostasis or salivary gland function in patients with HT. This study is the first to compare antioxidant defense and oxidative stress biomarkers in non-stimulated (NWS) and stimulated (SWS) whole saliva and plasma/erythrocytes of HT patients compared to controls. The study included 45 women with HT in the euthyreosis period as well as an age- and gender-matched control group. We showed that NWS secretion was significantly lower in HT patients compared to healthy controls, similar to salivary amylase activity in NWS and SWS. Catalase and peroxidase activities were considerably higher in NWS and SWS of HT patients, while the concentrations of reduced glutathione and uric acid were significantly lower in comparison with healthy subjects. Total antioxidant potential was significantly lower, while total oxidant status and the level of oxidation products of proteins (advanced glycation end products, advanced oxidation protein products) and lipids (malondialdehyde, lipid hydroperoxides) were significantly higher in NWS, SWS and plasma of HT patients. In conclusion, in both salivary glands of women with HT in euthyreosis, the ability to maintain redox homeostasis was hindered. In HT patients we observed oxidative damage to salivary proteins and lipids; thus, some biomarkers of oxidative stress may present a potential diagnostic value.
桥本甲状腺炎（HT）是最常见的自身免疫性疾病之一。尽管HT与氧化应激有着千丝万缕的联系，但尚无研究评估HT患者的唾液氧化还原稳态或唾液腺功能。本研究首次比较了HT患者与对照组在非刺激性（NWS）和刺激性（SWS）全唾液以及血浆/红细胞中的抗氧化防御和氧化应激生物标志物。该研究纳入了45名处于甲状腺功能正常期的HT女性患者以及一个年龄和性别匹配的对照组。我们发现，与健康对照组相比，HT患者的NWS分泌显著降低，NWS和SWS中的唾液淀粉酶活性也类似。HT患者的NWS和SWS中的过氧化氢酶和过氧化物酶活性明显更高，而与健康受试者相比，还原型谷胱甘肽和尿酸的浓度显著更低。HT患者的NWS、SWS和血浆中的总抗氧化能力显著更低，而总氧化剂状态以及蛋白质（晚期糖基化终产物、晚期氧化蛋白产物）和脂质（丙二醛、脂质氢过氧化物）的氧化产物水平显著更高。总之，处于甲状腺功能正常期的HT女性患者的两个唾液腺维持氧化还原稳态的能力均受到阻碍。在HT患者中，我们观察到唾液蛋白质和脂质受到氧化损伤；因此，一些氧化应激生物标志物可能具有潜在的诊断价值。
