Deng Xiaorong, Tian Huakai, Yang Rong, Han Yiwen, Wei Kehong, Zheng Cihua, Liu Zhaoxia, Chen Tingtao
Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, China.
National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University, Nanchang, China.
Front Med (Lausanne). 2020 Feb 28;7:73. doi: 10.3389/fmed.2020.00073. eCollection 2020.
Bowel preparation is necessary for successful colonoscopy, while it can seriously affect intestinal microbial composition and damage the intestinal mucosal barriers in humans. To figure out whether probiotics can sustain intestinal homeostasis and guard people's health, the probiotic drug of Tetragenous viable Bacteria Tablets (P group, = 16) or placebo (C group, = 16) was used for volunteers receiving bowel preparation, and high-throughput sequencing method was applied to monitor their intestinal microbial changes. The present results suggested that bowel preparation obviously reduced the intestinal microbial diversity, while taking probiotics significantly restored it to normal level. In addition, probiotics sharply reduced the abundance of pathogenic , and obviously lowered the ratio of compared with control group at phylum level ( < 0.05). And probiotics markedly decreased the abundance of pathogenic and , while greatly enriched the relative abundance of beneficial bacteria , and at genus level ( < 0.05). Probiotic drugs, e.g., Tetragenous viable Bacteria Tablets, can be used to restore intestinal dysbacteriosis caused by bowel preparation, and reduce side effects during colonoscopy.
肠道准备对于成功进行结肠镜检查是必要的，然而它会严重影响肠道微生物组成并损害人体肠道黏膜屏障。为了弄清楚益生菌是否能维持肠道稳态并保护人们的健康，将益生菌药物四联活菌片（P组，n = 16）或安慰剂（C组，n = 16）用于接受肠道准备的志愿者，并采用高通量测序方法监测他们的肠道微生物变化。目前的结果表明，肠道准备明显降低了肠道微生物多样性，而服用益生菌可将其显著恢复至正常水平。此外，与对照组相比，益生菌显著降低了病原菌的丰度，并且在门水平上明显降低了[具体菌门名称]的比例（P < 0.05）。在属水平上，益生菌显著降低了病原菌[具体菌属名称1]和[具体菌属名称2]的丰度，同时极大地增加了有益菌[具体菌属名称3]和[具体菌属名称4]的相对丰度（P < 0.05）。益生菌药物，如四联活菌片，可用于恢复由肠道准备引起的肠道菌群失调，并减少结肠镜检查期间的副作用。
