Svoronos Christos, Tsoulfas Georgios, Souvatzi Maria, Chatzitheoklitos Efthimios
Department of Surgery, AMEOS Klinikum, Halberstadt, Germany.
First Department of Surgery, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Ann Hepatobiliary Pancreat Surg. 2020 Feb;24(1):52-56. doi: 10.14701/ahbps.2020.24.1.52. Epub 2020 Feb 27.
BACKGROUNDS/AIMS: Pancreatic cancer is one of the most fatal human malignancies with poor prognosis, despite advances in therapy. Here, we evaluated the potential role of collagen type VI α3 chain (COL6A3) as a non-invasive biomarker for pancreatic adenocarcinoma.
In this study, we investigated immunohistochemically the expression of COL6A3 in 30 patients with resectable pancreatic adenocarcinoma by immunohistochemistry in a tissue sample of the cancer and a tissue sample of normal pancreas for each patient. Also, we looked for associations between COL6A3 and other prognostic factors of pancreatic cancer.
All of the pancreatic cancer tissue samples revealed in different ranges of intensity from weak (+) in 16.67%, moderate (+2) in 50%, to strongly positive (+3) in 33.33% staining for COL6A3. We found no moderate or strongly positive staining in normal pancreatic tissue. There was only weak positive staining in 23 samples (76.67%) and 7 (23.30%) were negative. Also, there was significant correlation between COL6A3 moderate and strongly expression and negative prognostic factors for pancreatic cancer.
The greatest density of COL6A3 was observed in pancreatic cancer tissues and was correlated with negative prognostic factors for pancreatic cancer. Therefore, we suggest that COL6A3 could be used as prognostic factor in pancreatic cancer, but more studies need to prove its value.
背景/目的：尽管治疗方法有所进步，但胰腺癌仍是预后最差的致命性人类恶性肿瘤之一。在此，我们评估了VI型胶原蛋白α3链（COL6A3）作为胰腺腺癌非侵入性生物标志物的潜在作用。
在本研究中，我们通过免疫组织化学方法，对30例可切除胰腺腺癌患者的癌组织样本和正常胰腺组织样本进行了COL6A3表达情况的研究。此外，我们还探寻了COL6A3与胰腺癌其他预后因素之间的关联。
所有胰腺癌组织样本中COL6A3染色强度呈现不同范围，16.67%为弱阳性（+），50%为中度阳性（+2），33.33%为强阳性（+3）。我们发现正常胰腺组织中无中度或强阳性染色。仅23个样本（76.67%）呈弱阳性，7个样本（23.30%）呈阴性。此外，COL6A3中度和强表达与胰腺癌的不良预后因素之间存在显著相关性。
在胰腺癌组织中观察到COL6A3密度最高，且与胰腺癌的不良预后因素相关。因此，我们认为COL6A3可作为胰腺癌的预后因素，但需要更多研究来证实其价值。
