Department of Endocrine and Metabolic Diseases, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China.
Aging (Albany NY). 2020 Mar 17;12(6):5071-5090. doi: 10.18632/aging.102932.
Aging induces gradual accumulation of damages in cells and tissues, which leads to physiological dysfunctions. Aging-associated muscle dysfunction is commonly seen in aged population and severely affects their physical activity and life quality, against which aerobic training has been shown to exert antagonizing or alleviating effects. Circular RNAs (circRNAs) play important roles in various physiological processes, yet their involvement in aging-associated muscle dysfunction is not well understood. In this study, we performed comprehensive analysis of circRNAs profiles in quadriceps muscles in sedentary young and aging mice, as well as aging mice with aerobic exercise using RNA sequencing. Our results identified circRNAs altered by factors of aging and aerobic exercise. Their host genes were then predicted and analyzed by gene ontology enrichment analysis. Importantly, we found that circBBS9 featured decreased levels in aging compared to young mice and elevated expression in exercise versus sedentary aging mice. Besides, we performed GO and KEGG analysis on circBBS9 target genes, as well as established the circBBS9-miRNA-mRNAs interaction network. Our results indicate that circBBS9 may play active roles in muscle aging by mediating the benefits of aerobic training intervention, thus may serve as a novel therapeutic target combating aging-associated muscle dysfunction.
衰老是导致细胞和组织损伤逐渐积累的原因，从而导致生理功能障碍。与衰老相关的肌肉功能障碍在老年人群中很常见，严重影响他们的身体活动和生活质量，而有氧运动已被证明具有对抗或缓解的作用。环状 RNA（circRNA）在各种生理过程中发挥着重要作用，但它们在与衰老相关的肌肉功能障碍中的作用尚不清楚。在这项研究中，我们使用 RNA 测序对久坐的年轻和衰老小鼠以及进行有氧运动的衰老小鼠的股四头肌中的 circRNA 谱进行了全面分析。我们的结果确定了受衰老和有氧运动因素影响的 circRNA。然后通过基因本体富集分析预测和分析它们的宿主基因。重要的是，我们发现与年轻小鼠相比，circBBS9 在衰老小鼠中的水平降低，而在运动小鼠中高于久坐衰老小鼠。此外，我们对 circBBS9 靶基因进行了 GO 和 KEGG 分析，并建立了 circBBS9-miRNA-mRNAs 相互作用网络。我们的结果表明，circBBS9 可能通过介导有氧运动干预的益处，在肌肉衰老中发挥积极作用，因此可能成为对抗与衰老相关的肌肉功能障碍的新的治疗靶点。
