Infectious and Tropical Diseases Group (e-INTRO). IBSAL-CIETUS (Biomedical Research Institute of Salamanca-Research Center for Tropical Diseases at the University of Salamanca), Faculty of Pharmacy, University of Salamanca, Salamanca, Spain.
Instituto de Investigación Biomédica de Salamanca (IBSAL), Universidad de Salamanca, CSIC, Complejo Asistencial Universitario de Salamanca, Salamanca, Spain.
Euro Surveill. 2020 Mar;25(10). doi: 10.2807/1560-7917.ES.2020.25.10.1900507.
BackgroundCrimean-Congo haemorrhagic fever virus (CCHFV) is considered an emerging or even a probable re-emerging pathogen in southern Europe. Presence of this virus had been reported previously in Spain in 2010.AimWe aimed to evaluate the potential circulation of CCHFV in western Spain with a serosurvey in asymptomatic adults (blood donors).MethodsDuring 2017 and 2018, we conducted a CCHFV serosurvey in randomly selected asymptomatic blood donors from western Spain. Three assays using specific IgG antibodies against CCHFV were performed: the VectoCrimea ELISA test, an in-house ELISA and indirect immunofluorescence (EuroImmun) test with glycoprotein and nucleoprotein.ResultsA total of 516 blood donors participated in this cross-sectional study. The majority of the study participants were male (68.4%), and the mean age was 46.3 years. Most of the participants came from rural areas (86.8%) and 68.6% had contact with animals and 20.9% had animal husbandry practices. One in five participants (109/516, 21.1%) were engaged in at-risk professional activities such as agriculture and shepherding, slaughtering, hunting, veterinary and healthcare work (mainly nursing staff and laboratory technicians). A total of 15.3% of the participants were bitten by ticks in the days or months before the date of sampling. We detected anti-CCHFV IgG antibodies with two diagnostic assays in three of the 516 individuals and with one diagnostic assay in six of the 516 individuals.ConclusionSeroprevalence of CCHFV was between 0.58% and 1.16% in Castile-León, Spain. This is the first study in western Spain that showed circulation of CCHFV in healthy people.
背景
克里米亚-刚果出血热病毒（CCHFV）被认为是一种在欧洲南部新出现的甚至可能重新出现的病原体。此前，西班牙曾于 2010 年报告过该病毒的存在。
目的
我们旨在评估 CCHFV 在西班牙西部的潜在传播情况，对无症状成年人（献血者）进行血清学调查。
方法
在 2017 年和 2018 年，我们对来自西班牙西部的随机选择的无症状献血者进行了 CCHFV 血清学调查。使用针对 CCHFV 的特异性 IgG 抗体进行了三种检测：VectoCrimea ELISA 检测、内部 ELISA 检测和间接免疫荧光（EuroImmun）检测，检测对象为糖蛋白和核蛋白。
结果
共有 516 名献血者参与了这项横断面研究。研究参与者中大多数为男性（68.4%），平均年龄为 46.3 岁。大多数参与者来自农村地区（86.8%），68.6%与动物接触，20.9%从事畜牧业。五分之一的参与者（109/516，21.1%）从事高风险的职业活动，如农业和放牧、屠宰、狩猎、兽医和医疗保健工作（主要是护理人员和实验室技术员）。在采样日期前的几天或几个月内，共有 15.3%的参与者被蜱虫叮咬。我们在 516 人中的 3 人用两种诊断检测方法检测到抗 CCHFV IgG 抗体，在 516 人中的 6 人用一种诊断检测方法检测到抗 CCHFV IgG 抗体。
结论
在西班牙卡斯蒂利亚-莱昂，CCHFV 的血清流行率在 0.58%至 1.16%之间。这是西班牙西部首次表明 CCHFV 在健康人群中传播的研究。
