Department of Cell and Developmental Biology, School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Proteomics Center, Institute of Biochemistry, Vilnius University Life Sciences Center, Vilnius 10257, Lithuania.
J Cell Sci. 2020 May 14;133(9):jcs241117. doi: 10.1242/jcs.241117.
During mitotic cell division, the actomyosin cytoskeleton undergoes several dynamic changes that play key roles in progression through mitosis. Although the regulators of cytokinetic ring formation and contraction are well established, proteins that regulate cortical stability during anaphase and telophase have been understudied. Here, we describe a role for CLIC4 in regulating actin and actin regulators at the cortex and cytokinetic cleavage furrow during cytokinesis. We first describe CLIC4 as a new component of the cytokinetic cleavage furrow that is required for successful completion of mitotic cell division. We also demonstrate that CLIC4 regulates the remodeling of the sub-plasma-membrane actomyosin network within the furrow by recruiting MST4 kinase (also known as STK26) and regulating ezrin phosphorylation. This work identifies and characterizes new molecular players involved in regulating cortex stiffness and blebbing during the late stages of cytokinetic furrowing.
在有丝分裂细胞分裂过程中，肌动球蛋白细胞骨架会经历几次动态变化，这些变化在有丝分裂进程中起着关键作用。虽然细胞分裂环形成和收缩的调节剂已经得到很好的确立，但在后期和末期调节皮质稳定性的蛋白质尚未得到充分研究。在这里，我们描述了 CLIC4 在调节有丝分裂细胞分裂过程中的皮质和胞质分裂沟中的肌动蛋白和肌动蛋白调节剂的作用。我们首先将 CLIC4 描述为胞质分裂沟中的一个新的组成部分，该部分对于成功完成有丝分裂细胞分裂是必需的。我们还证明，CLIC4 通过募集 MST4 激酶（也称为 STK26）并调节 ezrin 磷酸化来调节沟内亚质膜肌动球蛋白网络的重塑。这项工作鉴定并描述了新的分子参与者，它们在胞质分裂沟后期调节皮质硬度和起泡。
