Amare Gedefaw Getnet, Meharie Birhanu Geta, Belayneh Yaschilal Muche
Department of Pharmacy, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia.
Evid Based Complement Alternat Med. 2020 Oct 3;2020:8899743. doi: 10.1155/2020/8899743. eCollection 2020.
The leaf latex of has been used as remedy for diabetes mellitus. This was carried out to determine and antidiabetic activities of the leaf latex of a. . Sucrase and maltase inhibitory activity of the leaf latex of was determined in glucose oxidase assay, and -amylase inhibitory activity was determined in dinitrosalicylic acid assay. Normoglycemic, glucose-loaded, and streptozotocin-induced diabetic mice were treated orally to determine blood glucose lowering activity of the latex. Effect of the latex on serum lipid level and body weight was measured in streptozotocin-induced diabetic mice. Additionally, DPPH assay was used to determine free radical scavenging capacity of the latex. . Antioxidant activity of the latex was concentration dependent; the strongest inhibition was measured at 800 g/ml (80.57%). The leaf latex of inhibited sucrase (IC = 2.92 g/ml), maltase (IC = 11.81 g/ml) and -amylase (IC = 14.92 g/ml) enzymes. All doses of the leaf latex induced hypoglycemic effect after 4 h in normal mice, and low dose of the latex did not show significant effect after 6 h. Glucose reduction of the leaf latex of was significant ( < 0.05) in oral glucose-loaded mice compared to the vehicle control. Blood glucose level of diabetic mice was significantly ( < 0.05) reduced on week one and weak two in a streptozotocin-induced diabetic mouse model. Glucose reduction increased with increasing the doses of the leaf latex of on week one ( < 0.05 (200 mg/kg), < 0.01 (400 mg/kg), and < 0.001 (600 mg/kg)). Administration of the leaf latex of for two weeks significantly ( < 0.05) improved diabetic dyslipidemia and body weight of diabetic mice. . The study confirmed that the leaf latex of the plant showed a significant antidiabetic activity justifying the traditional uses of the plant.
某植物的叶乳胶已被用作糖尿病的治疗药物。本研究旨在确定该植物叶乳胶的抗糖尿病活性和抗氧化活性。通过葡萄糖氧化酶法测定该植物叶乳胶的蔗糖酶和麦芽糖酶抑制活性，通过二硝基水杨酸法测定α-淀粉酶抑制活性。对血糖正常、葡萄糖负荷及链脲佐菌素诱导的糖尿病小鼠进行口服给药，以确定乳胶的降血糖活性。在链脲佐菌素诱导的糖尿病小鼠中测量乳胶对血清脂质水平和体重的影响。此外，采用DPPH法测定乳胶的自由基清除能力。乳胶的抗氧化活性呈浓度依赖性；在800μg/ml时抑制作用最强（80.57%）。该植物叶乳胶抑制蔗糖酶（IC₅₀ = 2.92μg/ml）、麦芽糖酶（IC₅₀ = 11.81μg/ml）和α-淀粉酶（IC₅₀ = 14.92μg/ml）。所有剂量的叶乳胶在正常小鼠给药4小时后均诱导低血糖效应，低剂量乳胶在6小时后未显示显著效果。与赋形剂对照组相比，该植物叶乳胶在口服葡萄糖负荷小鼠中的葡萄糖降低具有显著性（P < 0.05）。在链脲佐菌素诱导的糖尿病小鼠模型中，糖尿病小鼠的血糖水平在第1周和第2周显著降低（P < 0.05）。在第1周，随着该植物叶乳胶剂量的增加，葡萄糖降低程度增加（P < 0.05（200mg/kg），P < 0.01（400mg/kg），P < 0.001（600mg/kg））。给予该植物叶乳胶两周显著改善了糖尿病小鼠的糖尿病血脂异常和体重（P < 0.05）。该研究证实，该植物的叶乳胶具有显著的抗糖尿病活性，证明了该植物的传统用途是合理的。
