Karimi Samaneh, Farzaneh Farzaneh, Asnaashari Solmaz, Parina Parina, Sarvari Yaser, Hazrati Saeid
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Pharmaceutical and Food Control, Faculty of Pharmacy, Tabriz University of Medical Sciences Tabriz, Iran.
Iran J Pharm Res. 2019 Fall;18(4):1871-1883. doi: 10.22037/ijpr.2019.1100817.
Due to the increase of microbial resistance to antibiotics and the occurrence of side effects, use of medicinal plants with anti-microbial properties seems to be rational. Hence, in this study, some plants of the Apiaceae, Asteraceae, Brassicaceae, and Cucurbitaceae families were evaluated for antimicrobial effects. The aerial parts of the plants were extracted by different solvents using a Soxhlet apparatus. Subsequently, the inhibitory effect of the extracts on different microbial species was assessed. Extracts with high growth inhibitory effect were fractionated and their MIC was determined. Furthermore, primary phytochemical and GC-MS analysis were used to identify the chemical compounds of potent samples of n-hexane extracts of () and (.) Both plants showed considerable antimicrobial activities against among the fractions, 40% and 60% VLC fractions of n-hex extract of and 40% VLC fraction of n-hexane extract of illustrated the most growth inhibitory effect. Moreover, the results of preliminary phytochemical and GC-MS analysis confirmed that steroids, fatty acids and terpenoids play an important role to show anti-microbial activity, respectively. Among all samples, the 40% VLC fraction of n-hexane extract of for possessing high amounts of fatty acids and terpenoids indicated the most anti-microbial potency.
由于微生物对抗生素的耐药性增加以及副作用的出现，使用具有抗菌特性的药用植物似乎是合理的。因此，在本研究中，对伞形科、菊科、十字花科和葫芦科的一些植物进行了抗菌效果评估。使用索氏提取器用不同溶剂提取植物的地上部分。随后，评估提取物对不同微生物物种的抑制作用。对具有高生长抑制作用的提取物进行分级分离并测定其最低抑菌浓度（MIC）。此外，采用初步植物化学分析和气相色谱 - 质谱联用（GC - MS）分析来鉴定（）和（.）正己烷提取物有效样品中的化合物。两种植物对均表现出相当的抗菌活性，在各馏分中，（植物名称）正己烷提取物的40%和60%体积排阻色谱（VLC）馏分以及（植物名称）正己烷提取物的40% VLC馏分显示出最强的生长抑制作用。此外，初步植物化学分析和GC - MS分析结果证实，类固醇、脂肪酸和萜类化合物分别在显示抗菌活性方面发挥重要作用。在所有样品中，（植物名称）正己烷提取物的40% VLC馏分由于含有大量脂肪酸和萜类化合物而显示出最强的抗菌效力。
