Molecular Medicine Research Center, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran.
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
BMC Complement Med Ther. 2022 May 14;22(1):134. doi: 10.1186/s12906-022-03618-w.
The genus Eryngium is a member of the Apiaceae family that has shown different pharmacological effects mainly including anti-inflammatory, analgesic, anti-cancer, hepatoprotective, and anti-oxidant. Previous research on the anti-cancer activity of Eryngium in some cancer cell lines has led us to explore the anti-proliferative activity of E. caucasicum in the B16F10 cell line.
In this study, the antiproliferative activity of E. caucasicum on melanoma cancer cells (B16F10) and non-cancerous cells (HFFF2) were evaluated in vitro.
The dried plant sample of E. caucasicum was extracted by Soxhlet apparatus with n-Hexane, dichloromethane, and methanol solvents. The effects of cytotoxicity of the extracts by the MTT method on melanoma cancer cells (B16F10) and noncancerous cells (HFFF2) was investigated for 24 and 48 hours. Then, the cytotoxicity of different fractions of the strong extract against normal and cancer cells was evaluated by this method. Annexin V/PI assay was used to study the induction apoptosis via the fractions in cancer cells.
According to the results of the MTT test, n-Hexane extract is the most effective extract against the B16F10 cell line and is a candidate for fractionation with VLC. Among the fractions, 40 and 60% VLC fractions of n-Hexan extract inhibited the growth of B16F10 cells at 24 and 48 hours while, these fractions at IC concentration had no cytotoxic effects on normal cells. Treatment of cancer cells with effective extract caused apoptosis and necrosis and 40 and 60% more fractions induced apoptosis in these cells.
The n-Hexane extract of E. caucasicum and its 40 and 60% fractions showed the highest cytotoxic effect against the B16F10 cell line compared to other extracts and control groups. This inhibition was made through induction of apoptosis.
蓝刺头属是伞形科的一个属，具有多种药理作用，主要包括抗炎、镇痛、抗癌、保肝和抗氧化作用。之前关于某些癌细胞系中蓝刺头属抗癌活性的研究促使我们探索蓝刺头属在 B16F10 细胞系中的抗增殖活性。
本研究评估了蓝刺头属植物干品提取物在体外对黑素瘤癌细胞（B16F10）和非癌细胞（HFFF2）的增殖抑制活性。
采用索氏提取器用正己烷、二氯甲烷和甲醇溶剂对蓝刺头属植物干品进行提取。采用 MTT 法研究提取物对黑素瘤癌细胞（B16F10）和非癌细胞（HFFF2）的细胞毒性，作用时间为 24 和 48 小时。然后，采用 MTT 法评估强提取物的不同馏分对正常细胞和癌细胞的细胞毒性。采用 Annexin V/PI 检测法研究各馏分在癌细胞中诱导凋亡的情况。
根据 MTT 试验的结果，正己烷提取物是对 B16F10 细胞系最有效的提取物，是用 VLC 进行分馏的候选物。在馏分中，正己烷提取物的 40%和 60%VLC 馏分在 24 和 48 小时时抑制 B16F10 细胞的生长，而在 IC 浓度下，这些馏分对正常细胞没有细胞毒性作用。用有效提取物处理癌细胞会导致细胞凋亡和坏死，40%和 60%的更多馏分会诱导这些细胞发生凋亡。
与其他提取物和对照组相比，蓝刺头属植物的正己烷提取物及其 40%和 60%馏分对 B16F10 细胞系表现出最高的细胞毒性作用。这种抑制是通过诱导细胞凋亡实现的。
