Shen Nengxing, Wei Wenrui, Chen Yuhang, Ren Yongjun, Xiong Lang, Tao Yuanyuan, Gu Xiaobin, Xie Yue, Peng Xuerong, Yang Guangyou
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China.
Animal Breeding and Genetics Key Laboratory of Sichuan Province, Chengdu 610066, Sichuan, China.
Vaccines (Basel). 2020 Mar 16;8(1):129. doi: 10.3390/vaccines8010129.
Scabies is a highly contagious disease caused by which burrows into stratum corneum of host's skin. In this study, after optimizing vaccination schedule, a vaccination trial is comprised of three test groups of rabbits ( = 10/group) by immunization with (1) rSsCLP5; (2) rSsCLP12; or (3) a mixture of rSsCLP5 and rSsCLP12, three biological replicates groups ( = 10/group) and three control groups ( = 10/group). Levels of specific IgG, total IgE and cytokines in sera were detected and histopathologically analyzed as indicators of vaccine effects. The results showed that 85% (17/20) of rabbits exhibited no detectable skin lesions of infestation in mixed protein groups compared to single protein groups with 75% (15/20) and 70% (14/20), respectively. Moreover, the deworming rates of mixed groups are increased by 10%-20% compared with that of single groups. Each of six groups immunized with rSsCLP displayed significant increases of specific IgG, total IgE, IL-10, and TNF-α. The degree of skin damage in test groups also significantly lower than that of control groups. Thus, purified rSsCLP5 and rSsCLP12 subunit cocktail vaccine induced robust immune protection and could significantly decrease mite populations to reduce the direct transmission between rabbits.
疥疮是一种由钻入宿主皮肤角质层的[病原体名称缺失]引起的高度传染性疾病。在本研究中，优化疫苗接种方案后，一项疫苗接种试验包括三组试验组兔子（每组n = 10），分别用(1) rSsCLP5；(2) rSsCLP12；或(3) rSsCLP5与rSsCLP12的混合物进行免疫接种，还有三个生物学重复组（每组n = 10）和三个对照组（每组n = 10）。检测血清中特异性IgG、总IgE和细胞因子水平，并进行组织病理学分析，作为疫苗效果的指标。结果显示，与单一蛋白组分别为75%（15/20）和70%（14/20）相比，混合蛋白组中85%（17/20）的兔子未出现可检测到的疥螨感染皮肤病变。此外，混合组的驱虫率比单一组提高了10%-20%。用rSsCLP免疫的六组兔子中，每组的特异性IgG、总IgE、IL-10和TNF-α均显著增加。试验组的皮肤损伤程度也显著低于对照组。因此，纯化的rSsCLP5和rSsCLP12亚单位联合疫苗诱导了强大的免疫保护作用，并能显著减少螨的数量，以减少兔子之间的直接传播。
