Key Lab of Eco-restoration of Regional Contaminated Environment (Shenyang University), Ministry of Education, Shenyang 110044, China.
Department of Tissue Engineering, China Medical University, Shenyang 110122, China.
Molecules. 2020 Mar 17;25(6):1356. doi: 10.3390/molecules25061356.
Sustainable production of secondary metabolites in medicinal plants by artificial culturing on the industrial scale has gained worldwide importance. Engineered nanoparticles (ENPs) play a pivotal role in the elicitation of compounds of medicinal value. This investigation explores the influence of ZnO and CuO ENPs on in vitro roots formation, non-enzymatic antioxidant activities, and production of steviol glycosides (SGs) in regenerants of Candyleaf, . ENPs were applied in 0, 2, 20, 200, and 2000 mg/L of concentration in the MS medium containing plant shoots. The percentage of rooting induced was 91% and 94% by applying ZnO ENPs (2 mg/L) and CuO ENPs (20 mg/L), respectively. Moreover, at 2 mg/L of ZnO and 20 mg/L of CuO ENPs, the high performance liquid chromatography studies determined the significantly greatest content of SGs; rebaudioside A (4.42 and 4.44) and stevioside (1.28 and 1.96). Phytochemical studies including total flavonoid content, total phenolic content, and 2,2-diphenyl-1-picryl hydrazyl-free radical scavenging activity were calculated highest by the regenerants grown in 2 mg/L of ZnO and 20 mg/L of CuO ENPs dosage. Both ZnO and CuO ENPs at 200 mg/L and 2000 mg/L of concentration induced adverse effects on plant biomass, antioxidant activities, and SGs content up to 1.22 and 1.77 for rebaudioside A and 0.21 and 0.25 for stevioside. Hence, the biochemical and morphophysiological responses of Candyleaf were elicited as a defense against ZnO and CuO ENPs applied under threshold limit. This artificial biotechnological technique holds great promise for continued production of natural antioxidants on commercial scale and our study has further strengthened this impact.
在工业规模上通过人工培养生产药用植物中的次生代谢产物已引起全球关注。工程纳米粒子（ENP）在诱导具有药用价值的化合物方面起着关键作用。本研究探讨了 ZnO 和 CuO ENP 对 Candyleaf 离体根形成、非酶抗氧化活性和甜菊糖苷（SG）生产的影响。在含有植物芽的 MS 培养基中，将 ENP 以 0、2、20、200 和 2000 mg/L 的浓度添加。用 ZnO ENP（2 mg/L）和 CuO ENP（20 mg/L）分别诱导 91%和 94%的生根率。此外，在 2 mg/L 的 ZnO 和 20 mg/L 的 CuO ENP 下，高效液相色谱研究确定 SG 含量显著最高；瑞鲍迪甙 A（4.42 和 4.44）和甜菊糖苷（1.28 和 1.96）。用 2 mg/L 的 ZnO 和 20 mg/L 的 CuO ENP 培养的再生体的总黄酮含量、总酚含量和 2,2-二苯基-1-苦基肼自由基清除活性最高。在 200 mg/L 和 2000 mg/L 的浓度下，ZnO 和 CuO ENP 对植物生物量、抗氧化活性和 SG 含量产生不利影响，瑞鲍迪甙 A 为 1.22 和 1.77，甜菊糖苷为 0.21 和 0.25。因此，Candyleaf 的生化和形态生理学反应被激发为对施加的 ZnO 和 CuO ENP 的防御，施加的浓度在阈值限制内。这种人工生物技术技术有望在商业规模上继续生产天然抗氧化剂，我们的研究进一步加强了这一影响。
